Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-MazE
Location 2123649..2124299 Replicon chromosome
Accession NZ_CP017499
Organism Mannheimia haemolytica strain 33702

Toxin (Protein)


Gene name vapC Uniprot ID A0A378NBZ4
Locus tag BG576_RS10695 Protein ID WP_020831127.1
Coordinates 2123649..2124038 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1D2Q6P1
Locus tag BG576_RS10700 Protein ID WP_006251766.1
Coordinates 2124039..2124299 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG576_RS10655 2119210..2120019 - 810 WP_006250123.1 metal ABC transporter permease -
BG576_RS10660 2120012..2120872 - 861 WP_006250124.1 metal ABC transporter permease -
BG576_RS10665 2120946..2121731 - 786 WP_006251768.1 hypothetical protein -
BG576_RS10690 2122391..2123629 - 1239 WP_006251767.1 peptidase T -
BG576_RS10695 2123649..2124038 - 390 WP_020831127.1 type II toxin-antitoxin system VapC family toxin Toxin
BG576_RS10700 2124039..2124299 - 261 WP_006251766.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BG576_RS10705 2124415..2124627 - 213 WP_006251765.1 BrnA antitoxin family protein -
BG576_RS10710 2124628..2124915 - 288 WP_020831126.1 BrnT family toxin -
BG576_RS10715 2125078..2128611 - 3534 WP_006251763.1 transcription-repair coupling factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-119)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14575.87 Da        Isoelectric Point: 7.7391

>T67020 WP_020831127.1 NZ_CP017499:c2124038-2123649 [Mannheimia haemolytica]
MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYGVAKRNNGQLSQFLGYFLSSVKVLDWNYRCAELYGKL
RAEMEKNGKVMGVQDQMIASHALAEECILVSSDQAFKMVPDLKLENWLH

Download         Length: 390 bp

>T67020 NZ_CP017499:c2124038-2123649 [Mannheimia haemolytica]
ATGTATATGTTAGATACGAATACTGTAAGCTATTTTTTTCGTGGAAATGCAAATGTGGTGGCGAAATTGAAGTCCATTAA
TCCTGAGAGGCTTTGTATTTCATCTGTAACTGCTGCTGAATTGGTTTATGGTGTGGCGAAAAGAAATAATGGGCAGCTTT
CTCAATTTTTAGGTTATTTTTTATCGAGTGTAAAGGTTTTAGATTGGAATTATCGTTGTGCTGAACTTTATGGAAAGTTA
CGGGCAGAGATGGAGAAAAATGGCAAAGTAATGGGCGTGCAGGATCAAATGATTGCTAGTCACGCTCTTGCTGAAGAGTG
TATTTTAGTTTCTAGCGATCAGGCATTTAAAATGGTACCTGATCTGAAATTGGAGAATTGGTTACACTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 10245.50 Da        Isoelectric Point: 4.6909

>AT67020 WP_006251766.1 NZ_CP017499:c2124299-2124039 [Mannheimia haemolytica]
MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKQENGDILLSLRSRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQR
DPFEVA

Download         Length: 261 bp

>AT67020 NZ_CP017499:c2124299-2124039 [Mannheimia haemolytica]
ATGGAAAAAATTGCGAGATTATTCCGTAACGGGCGGAACCAAGCAGTGCGTTTACCGGTTGAATTTGAGTTTGATGCAGA
GCAAGTCTATGTTTCTAAACAAGAAAATGGCGATATTTTATTATCATTGAGATCGAGAAATACCGAGCATTGGCAACGAT
TGTTTGAGCTATTGCCTGCTGTTGTATGTGATGAAACATTTTTAGATACAAAAGAGAGAAACCAATCCTTTGAGCAACGG
GATCCTTTTGAGGTAGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NBZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A1D2Q6P1

References