Detailed information of TA system TA067016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 2492833..2493548
• Replicon: chromosome
• Accession: NZ_CP017498
• Organism: Mannheimia haemolytica strain 33980

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BG577_RS12545
• Protein ID: WP_006250491.1
• Coordinates: 2493120..2493548 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A248ZW87
• Locus tag: BG577_RS12540
• Protein ID: WP_006248272.1
• Coordinates: 2492833..2493120 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG577_RS12520	2488508..2488564	-	57	WP_075271894.1	PT domain-containing protein	-
BG577_RS12525	2488672..2490387	-	1716	WP_006248268.1	proline--tRNA ligase	-
BG577_RS12530	2490551..2490733	-	183	WP_006250493.1	hypothetical protein	-
BG577_RS12535	2490786..2492669	-	1884	WP_006250492.1	molecular chaperone HtpG	-
BG577_RS12540	2492833..2493120	+	288	WP_006248272.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BG577_RS12545	2493120..2493548	+	429	WP_006250491.1	type II toxin-antitoxin system VapC family toxin	Toxin
BG577_RS12550	2493552..2495633	+	2082	WP_006250490.1	ATP-dependent DNA helicase RecG	-
BG577_RS12555	2495655..2496587	-	933	WP_006250489.1	nucleoside hydrolase	-
BG577_RS12560	2496640..2498292	-	1653	WP_006250488.1	phospho-sugar mutase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 16640.06 Da
• Isoelectric Point: 8.9269

>T67016 WP_006250491.1 NZ_CP017498:2493120-2493548 [Mannheimia haemolytica]
MYRYLFDTNIVSELYKLRNDKMDVNVRQWLRGVNPSQTNISCITLSEIKTGILLKARKDKEQSDRLNDWFENNVLKAYQT
KAFVVNNDIALLASEYHIPNKMDLNDAYIAATAKYHNLILVTRNTKDFIRCDVRLFNPFETA

Nucleotide

• Length: 429 bp

>T67016 NZ_CP017498:2493120-2493548 [Mannheimia haemolytica]
ATGTATCGCTACCTTTTTGATACCAATATAGTTTCCGAGCTTTATAAGTTAAGAAATGACAAGATGGATGTCAATGTTCG
CCAGTGGCTGAGAGGGGTTAATCCTAGCCAGACCAATATCAGCTGTATTACGTTATCTGAAATCAAAACAGGTATTTTGC
TGAAAGCCCGCAAAGACAAAGAACAATCAGACCGATTAAATGATTGGTTTGAAAATAATGTATTAAAGGCTTATCAAACA
AAAGCGTTTGTGGTGAATAACGACATTGCTCTATTGGCATCAGAGTACCATATTCCCAATAAAATGGATTTAAATGATGC
TTATATTGCGGCAACCGCCAAATACCACAATCTGATTTTAGTCACCCGCAACACCAAAGATTTTATCCGTTGTGATGTGC
GTTTATTTAACCCATTTGAAACCGCATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11085.39 Da
• Isoelectric Point: 4.3648

>AT67016 WP_006248272.1 NZ_CP017498:2492833-2493120 [Mannheimia haemolytica]
MAITITSREFNQNASKVLKMAEKEPVFITKWGKIVNVVSSYMDYQQKKPSEPSFEELFASSENEDVSDEFWQEFEQTLVE
IRKSNKPRPVDFGDE

Nucleotide

• Length: 288 bp

>AT67016 NZ_CP017498:2492833-2493120 [Mannheimia haemolytica]
ATGGCAATAACCATTACTTCCAGAGAATTTAATCAAAATGCCAGCAAAGTGTTAAAAATGGCAGAAAAAGAGCCGGTTTT
TATTACCAAATGGGGCAAAATCGTCAATGTGGTGTCTAGTTATATGGATTATCAGCAGAAAAAGCCTAGCGAGCCAAGCT
TTGAAGAGCTATTCGCCTCTTCCGAAAATGAGGATGTGAGCGATGAATTTTGGCAAGAGTTTGAACAAACATTGGTTGAA
ATCCGCAAAAGCAATAAACCAAGACCTGTTGATTTTGGAGATGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A248ZW87