Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1981996..1982636 Replicon chromosome
Accession NZ_CP017498
Organism Mannheimia haemolytica strain 33980

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG577_RS09795 Protein ID WP_006250075.1
Coordinates 1981996..1982178 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NET2
Locus tag BG577_RS09800 Protein ID WP_006250984.1
Coordinates 1982217..1982636 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG577_RS09760 1977567..1978613 - 1047 WP_020824125.1 conserved phage C-terminal domain-containing protein -
BG577_RS09765 1978610..1979449 - 840 WP_006252350.1 Rha family transcriptional regulator -
BG577_RS09770 1979625..1979885 - 261 WP_006252483.1 hypothetical protein -
BG577_RS09775 1979906..1980106 - 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG577_RS09780 1980237..1980920 + 684 WP_006252485.1 helix-turn-helix transcriptional regulator -
BG577_RS09785 1980995..1981375 + 381 WP_006248823.1 hypothetical protein -
BG577_RS09790 1981380..1981865 + 486 WP_006252741.1 hypothetical protein -
BG577_RS09795 1981996..1982178 + 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG577_RS09800 1982217..1982636 + 420 WP_006250984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG577_RS09805 1982703..1983008 - 306 WP_006250985.1 HigA family addiction module antidote protein -
BG577_RS09810 1983017..1983292 - 276 WP_006250986.1 type II toxin-antitoxin system RelE/ParE family toxin -
BG577_RS09815 1983582..1983812 + 231 WP_006250987.1 hypothetical protein -
BG577_RS09820 1984291..1984614 - 324 WP_006250988.1 hypothetical protein -
BG577_RS09825 1984807..1984992 + 186 WP_020824222.1 hypothetical protein -
BG577_RS12765 1984976..1985140 + 165 WP_020824223.1 hypothetical protein -
BG577_RS09830 1985146..1985604 + 459 WP_006253113.1 single-stranded DNA-binding protein -
BG577_RS09835 1985640..1985999 + 360 WP_006250991.1 hypothetical protein -
BG577_RS09840 1986071..1986874 + 804 WP_020824224.1 DUF2303 family protein -
BG577_RS09845 1986968..1987402 + 435 WP_020824225.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1921006..1992984 71978


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T67014 WP_006250075.1 NZ_CP017498:1981996-1982178 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T67014 NZ_CP017498:1981996-1982178 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15957.34 Da        Isoelectric Point: 4.6983

>AT67014 WP_006250984.1 NZ_CP017498:1982217-1982636 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH

Download         Length: 420 bp

>AT67014 NZ_CP017498:1982217-1982636 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATACCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGTAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACGCT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NET2

References