Detailed information of TA system TA067008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2302105..2302753
• Replicon: chromosome
• Accession: NZ_CP017497
• Organism: Mannheimia haemolytica strain 34142

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A249A099
• Locus tag: BG547_RS11600
• Protein ID: WP_006251221.1
• Coordinates: 2302105..2302281 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A248ZZL6
• Locus tag: BG547_RS11605
• Protein ID: WP_006251222.1
• Coordinates: 2302337..2302753 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG547_RS11575	2297417..2299912	-	2496	WP_061888644.1	phage tail length tape measure family protein	-
BG547_RS11580	2299999..2300394	-	396	WP_006251217.1	hypothetical protein	-
BG547_RS11585	2300462..2300920	-	459	WP_006251218.1	hypothetical protein	-
BG547_RS11590	2301089..2301322	+	234	WP_020824316.1	hypothetical protein	-
BG547_RS11595	2301337..2302008	-	672	WP_006251220.1	hypothetical protein	-
BG547_RS11600	2302105..2302281	+	177	WP_006251221.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG547_RS11605	2302337..2302753	+	417	WP_006251222.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG547_RS11610	2302793..2303275	-	483	WP_006251223.1	phage tail protein	-
BG547_RS11615	2303279..2303659	-	381	WP_006251224.1	hypothetical protein	-
BG547_RS11620	2303656..2304024	-	369	WP_006251225.1	hypothetical protein	-
BG547_RS11625	2304026..2304370	-	345	WP_006251226.1	hypothetical protein	-
BG547_RS11630	2304370..2304747	-	378	WP_006251227.1	hypothetical protein	-
BG547_RS11635	2304750..2305001	-	252	WP_015484272.1	hypothetical protein	-
BG547_RS11640	2305013..2305999	-	987	WP_062627922.1	encapsulin	-
BG547_RS11645	2306014..2306448	-	435	WP_006251230.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2288431..2351247	62816

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6640.79 Da
• Isoelectric Point: 10.7708

>T67008 WP_006251221.1 NZ_CP017497:2302105-2302281 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Nucleotide

• Length: 177 bp

>T67008 NZ_CP017497:2302105-2302281 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15519.91 Da
• Isoelectric Point: 4.3508

>AT67008 WP_006251222.1 NZ_CP017497:2302337-2302753 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Nucleotide

• Length: 417 bp

>AT67008 NZ_CP017497:2302337-2302753 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Toxin

• AlphaFold DB: A0A249A099

Antitoxin

• AlphaFold DB: A0A248ZZL6