Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1961394..1962034 Replicon chromosome
Accession NZ_CP017497
Organism Mannheimia haemolytica strain 34142

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG547_RS09730 Protein ID WP_006250075.1
Coordinates 1961394..1961576 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NET2
Locus tag BG547_RS09735 Protein ID WP_006250984.1
Coordinates 1961615..1962034 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG547_RS09695 1956965..1958011 - 1047 WP_020824125.1 conserved phage C-terminal domain-containing protein -
BG547_RS09700 1958008..1958847 - 840 WP_006252350.1 Rha family transcriptional regulator -
BG547_RS09705 1959023..1959283 - 261 WP_006252483.1 hypothetical protein -
BG547_RS09710 1959304..1959504 - 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG547_RS09715 1959635..1960318 + 684 WP_006252485.1 helix-turn-helix transcriptional regulator -
BG547_RS09720 1960393..1960773 + 381 WP_006248823.1 hypothetical protein -
BG547_RS09725 1960778..1961263 + 486 WP_006252741.1 hypothetical protein -
BG547_RS09730 1961394..1961576 + 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG547_RS09735 1961615..1962034 + 420 WP_006250984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG547_RS09740 1962101..1962406 - 306 WP_006250985.1 HigA family addiction module antidote protein -
BG547_RS09745 1962415..1962690 - 276 WP_006250986.1 type II toxin-antitoxin system RelE/ParE family toxin -
BG547_RS09750 1962980..1963210 + 231 WP_006250987.1 hypothetical protein -
BG547_RS09755 1963689..1964012 - 324 WP_006250988.1 hypothetical protein -
BG547_RS09760 1964205..1964390 + 186 WP_020824222.1 hypothetical protein -
BG547_RS13360 1964374..1964538 + 165 WP_020824223.1 hypothetical protein -
BG547_RS09765 1964544..1965002 + 459 WP_006253113.1 single-stranded DNA-binding protein -
BG547_RS09770 1965038..1965397 + 360 WP_006250991.1 hypothetical protein -
BG547_RS09775 1965469..1966272 + 804 WP_020824224.1 DUF2303 family protein -
BG547_RS09780 1966366..1966800 + 435 WP_020824225.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1890708..1972382 81674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T67006 WP_006250075.1 NZ_CP017497:1961394-1961576 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T67006 NZ_CP017497:1961394-1961576 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15957.34 Da        Isoelectric Point: 4.6983

>AT67006 WP_006250984.1 NZ_CP017497:1961615-1962034 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH

Download         Length: 420 bp

>AT67006 NZ_CP017497:1961615-1962034 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATACCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGTAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACGCT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NET2

References