Detailed information of TA system TA067001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2292326..2292974
• Replicon: chromosome
• Accession: NZ_CP017496
• Organism: Mannheimia haemolytica strain 37121

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A249A099
• Locus tag: BG557_RS11690
• Protein ID: WP_006251221.1
• Coordinates: 2292798..2292974 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A248ZZL6
• Locus tag: BG557_RS11685
• Protein ID: WP_006251222.1
• Coordinates: 2292326..2292742 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG557_RS11645	2288649..2289083	+	435	WP_006251230.1	hypothetical protein	-
BG557_RS11650	2289098..2290084	+	987	WP_062627922.1	encapsulin	-
BG557_RS11655	2290096..2290329	+	234	WP_075271287.1	hypothetical protein	-
BG557_RS11660	2290332..2290709	+	378	WP_006251227.1	hypothetical protein	-
BG557_RS11665	2290709..2291053	+	345	WP_006251226.1	hypothetical protein	-
BG557_RS11670	2291055..2291423	+	369	WP_006251225.1	hypothetical protein	-
BG557_RS11675	2291420..2291800	+	381	WP_006251224.1	hypothetical protein	-
BG557_RS11680	2291804..2292286	+	483	WP_006251223.1	phage tail protein	-
BG557_RS11685	2292326..2292742	-	417	WP_006251222.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG557_RS11690	2292798..2292974	-	177	WP_006251221.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG557_RS11695	2293071..2293742	+	672	WP_006251220.1	hypothetical protein	-
BG557_RS11700	2293757..2294026	-	270	WP_006251219.1	hypothetical protein	-
BG557_RS11705	2294159..2294617	+	459	WP_006251218.1	hypothetical protein	-
BG557_RS11710	2294685..2295080	+	396	WP_006251217.1	hypothetical protein	-
BG557_RS11715	2295167..2297656	+	2490	WP_147009468.1	phage tail length tape measure family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2255866..2306642	50776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6640.79 Da
• Isoelectric Point: 10.7708

>T67001 WP_006251221.1 NZ_CP017496:c2292974-2292798 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Nucleotide

• Length: 177 bp

>T67001 NZ_CP017496:c2292974-2292798 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15519.91 Da
• Isoelectric Point: 4.3508

>AT67001 WP_006251222.1 NZ_CP017496:c2292742-2292326 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Nucleotide

• Length: 417 bp

>AT67001 NZ_CP017496:c2292742-2292326 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Toxin

• AlphaFold DB: A0A249A099

Antitoxin

• AlphaFold DB: A0A248ZZL6