Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1958656..1959296 Replicon chromosome
Accession NZ_CP017496
Organism Mannheimia haemolytica strain 37121

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG557_RS09690 Protein ID WP_006250075.1
Coordinates 1958656..1958838 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NET2
Locus tag BG557_RS09695 Protein ID WP_006250984.1
Coordinates 1958877..1959296 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG557_RS09655 1954227..1955273 - 1047 WP_020824125.1 conserved phage C-terminal domain-containing protein -
BG557_RS09660 1955270..1956109 - 840 WP_006252350.1 Rha family transcriptional regulator -
BG557_RS09665 1956285..1956545 - 261 WP_006252483.1 hypothetical protein -
BG557_RS09670 1956566..1956766 - 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG557_RS09675 1956897..1957580 + 684 WP_006252485.1 helix-turn-helix transcriptional regulator -
BG557_RS09680 1957655..1958035 + 381 WP_006248823.1 hypothetical protein -
BG557_RS09685 1958040..1958525 + 486 WP_186805239.1 hypothetical protein -
BG557_RS09690 1958656..1958838 + 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG557_RS09695 1958877..1959296 + 420 WP_006250984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG557_RS09700 1959363..1959668 - 306 WP_006250985.1 HigA family addiction module antidote protein -
BG557_RS09705 1959677..1959952 - 276 WP_006250986.1 type II toxin-antitoxin system RelE/ParE family toxin -
BG557_RS09710 1960242..1960472 + 231 WP_006250987.1 hypothetical protein -
BG557_RS09715 1960951..1961274 - 324 WP_006250988.1 hypothetical protein -
BG557_RS09720 1961467..1961652 + 186 WP_020824222.1 hypothetical protein -
BG557_RS13365 1961636..1961800 + 165 WP_020824223.1 hypothetical protein -
BG557_RS09725 1961806..1962264 + 459 WP_006253113.1 single-stranded DNA-binding protein -
BG557_RS09730 1962300..1962659 + 360 WP_006250991.1 hypothetical protein -
BG557_RS09735 1962731..1963534 + 804 WP_020824224.1 DUF2303 family protein -
BG557_RS09740 1963628..1964062 + 435 WP_020824225.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1916097..1969147 53050


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T66999 WP_006250075.1 NZ_CP017496:1958656-1958838 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T66999 NZ_CP017496:1958656-1958838 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15957.34 Da        Isoelectric Point: 4.6983

>AT66999 WP_006250984.1 NZ_CP017496:1958877-1959296 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH

Download         Length: 420 bp

>AT66999 NZ_CP017496:1958877-1959296 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATACCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGTAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACGCT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NET2

References