Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 804094..804616 Replicon chromosome
Accession NZ_CP017495
Organism Mannheimia haemolytica strain 37778

Toxin (Protein)


Gene name - Uniprot ID A0A249A107
Locus tag BG548_RS04060 Protein ID WP_006249269.1
Coordinates 804094..804390 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A378NDF7
Locus tag BG548_RS04065 Protein ID WP_006250574.1
Coordinates 804374..804616 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG548_RS04045 799149..800474 + 1326 WP_020831251.1 deoxyguanosinetriphosphate triphosphohydrolase family protein -
BG548_RS04050 800595..800894 + 300 WP_006249265.1 hypothetical protein -
BG548_RS04055 801055..803967 + 2913 WP_006250573.1 RNA polymerase-associated protein RapA -
BG548_RS04060 804094..804390 + 297 WP_006249269.1 BrnT family toxin Toxin
BG548_RS04065 804374..804616 + 243 WP_006250574.1 hypothetical protein Antitoxin
BG548_RS04070 804628..805296 + 669 WP_006249271.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
BG548_RS04075 805274..805849 - 576 WP_006249272.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
BG548_RS04080 805909..806751 + 843 WP_006250575.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
BG548_RS04085 806874..807515 + 642 WP_006250576.1 orotate phosphoribosyltransferase -
BG548_RS04090 807689..808766 + 1078 Protein_783 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(15-90)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 12009.89 Da        Isoelectric Point: 7.9454

>T66991 WP_006249269.1 NZ_CP017495:804094-804390 [Mannheimia haemolytica]
MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKVVLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRL
ISVRRSRKKEISYYEKYH

Download         Length: 297 bp

>T66991 NZ_CP017495:804094-804390 [Mannheimia haemolytica]
ATGTATATAGATTTGCAATATGCTGTGCCGTTATTGTTTGAATACGACCCGAACAAAAGCCAAATTAATTTAGCCAAATA
TGGTATTGATTTTGAACAGGCAAAGTTGCTTTGGGAAGATGAACGAAAAGTTGTACTGGAGGCTTGCATTGAACCCGAAT
TACGCTATTTCCTGATTGGCAAGATTTATGATAAATATTGGACAGCGTTTTTTACACCAAGAAATGATGTTATTCGTTTA
ATTTCAGTTAGACGTTCCCGTAAAAAAGAGATCTCATACTATGAGAAATACCATTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9320.71 Da        Isoelectric Point: 7.1665

>AT66991 WP_006250574.1 NZ_CP017495:804374-804616 [Mannheimia haemolytica]
MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFPNWMVEALDKQARHLGVPRQALSKMWIAEKLPVQSPR

Download         Length: 243 bp

>AT66991 NZ_CP017495:804374-804616 [Mannheimia haemolytica]
ATGAGAAATACCATTAGTGCCGAAGAATTAGAACGTATCTTCGATGAAGGCAAAGAAGATATTTTACAATACTGCGATCT
TTCTACCGCTCATCGCCCGGGGCAAATGAAAAGGGTGAATGTTGATTTCCCAAATTGGATGGTAGAAGCGTTAGATAAGC
AAGCCAGACATTTAGGTGTGCCTCGCCAAGCCTTGAGTAAAATGTGGATTGCGGAAAAATTACCGGTTCAATCACCTAGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A107

Antitoxin

Source ID Structure
AlphaFold DB A0A378NDF7

References