Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1994549..1995189 Replicon chromosome
Accession NZ_CP017493
Organism Mannheimia haemolytica strain 38120

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG568_RS09870 Protein ID WP_006250075.1
Coordinates 1994549..1994731 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NET2
Locus tag BG568_RS09875 Protein ID WP_006250984.1
Coordinates 1994770..1995189 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG568_RS09835 1990120..1991166 - 1047 WP_020824125.1 conserved phage C-terminal domain-containing protein -
BG568_RS09840 1991163..1992002 - 840 WP_006252350.1 Rha family transcriptional regulator -
BG568_RS09845 1992178..1992438 - 261 WP_006252483.1 hypothetical protein -
BG568_RS09850 1992459..1992659 - 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG568_RS09855 1992790..1993473 + 684 WP_006252485.1 helix-turn-helix transcriptional regulator -
BG568_RS09860 1993548..1993928 + 381 WP_006248823.1 hypothetical protein -
BG568_RS09865 1993933..1994418 + 486 WP_006252741.1 hypothetical protein -
BG568_RS09870 1994549..1994731 + 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG568_RS09875 1994770..1995189 + 420 WP_006250984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG568_RS09880 1995256..1995561 - 306 WP_006250985.1 HigA family addiction module antidote protein -
BG568_RS09885 1995570..1995845 - 276 WP_006250986.1 type II toxin-antitoxin system RelE/ParE family toxin -
BG568_RS09890 1996135..1996365 + 231 WP_006250987.1 hypothetical protein -
BG568_RS09895 1996844..1997167 - 324 WP_006250988.1 hypothetical protein -
BG568_RS09900 1997360..1997545 + 186 WP_020824222.1 hypothetical protein -
BG568_RS13345 1997529..1997693 + 165 WP_020824223.1 hypothetical protein -
BG568_RS09905 1997699..1998157 + 459 WP_006253113.1 single-stranded DNA-binding protein -
BG568_RS09910 1998193..1998552 + 360 WP_006250991.1 hypothetical protein -
BG568_RS09915 1998624..1999427 + 804 WP_020824224.1 DUF2303 family protein -
BG568_RS09920 1999479..1999913 + 435 WP_020824225.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1951990..2004998 53008


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T66978 WP_006250075.1 NZ_CP017493:1994549-1994731 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T66978 NZ_CP017493:1994549-1994731 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15957.34 Da        Isoelectric Point: 4.6983

>AT66978 WP_006250984.1 NZ_CP017493:1994770-1995189 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH

Download         Length: 420 bp

>AT66978 NZ_CP017493:1994770-1995189 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATACCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGTAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACGCT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NET2

References