Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 840431..840953 Replicon chromosome
Accession NZ_CP017493
Organism Mannheimia haemolytica strain 38120

Toxin (Protein)


Gene name - Uniprot ID A0A249A107
Locus tag BG568_RS04235 Protein ID WP_006249269.1
Coordinates 840431..840727 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A378NDF7
Locus tag BG568_RS04240 Protein ID WP_006250574.1
Coordinates 840711..840953 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG568_RS04220 835486..836811 + 1326 WP_020831251.1 deoxyguanosinetriphosphate triphosphohydrolase family protein -
BG568_RS04225 836932..837231 + 300 WP_006249265.1 hypothetical protein -
BG568_RS04230 837392..840304 + 2913 WP_006250573.1 RNA polymerase-associated protein RapA -
BG568_RS04235 840431..840727 + 297 WP_006249269.1 BrnT family toxin Toxin
BG568_RS04240 840711..840953 + 243 WP_006250574.1 hypothetical protein Antitoxin
BG568_RS04245 840965..841633 + 669 WP_006249271.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
BG568_RS04250 841611..842186 - 576 WP_006249272.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
BG568_RS04255 842246..843088 + 843 WP_006250575.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
BG568_RS04260 843211..843852 + 642 WP_006250576.1 orotate phosphoribosyltransferase -
BG568_RS04265 844026..845103 + 1078 Protein_813 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(15-90)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 12009.89 Da        Isoelectric Point: 7.9454

>T66977 WP_006249269.1 NZ_CP017493:840431-840727 [Mannheimia haemolytica]
MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKVVLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRL
ISVRRSRKKEISYYEKYH

Download         Length: 297 bp

>T66977 NZ_CP017493:840431-840727 [Mannheimia haemolytica]
ATGTATATAGATTTGCAATATGCTGTGCCGTTATTGTTTGAATACGACCCGAACAAAAGCCAAATTAATTTAGCCAAATA
TGGTATTGATTTTGAACAGGCAAAGTTGCTTTGGGAAGATGAACGAAAAGTTGTACTGGAGGCTTGCATTGAACCCGAAT
TACGCTATTTCCTGATTGGCAAGATTTATGATAAATATTGGACAGCGTTTTTTACACCAAGAAATGATGTTATTCGTTTA
ATTTCAGTTAGACGTTCCCGTAAAAAAGAGATCTCATACTATGAGAAATACCATTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9320.71 Da        Isoelectric Point: 7.1665

>AT66977 WP_006250574.1 NZ_CP017493:840711-840953 [Mannheimia haemolytica]
MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFPNWMVEALDKQARHLGVPRQALSKMWIAEKLPVQSPR

Download         Length: 243 bp

>AT66977 NZ_CP017493:840711-840953 [Mannheimia haemolytica]
ATGAGAAATACCATTAGTGCCGAAGAATTAGAACGTATCTTCGATGAAGGCAAAGAAGATATTTTACAATACTGCGATCT
TTCTACCGCTCATCGCCCGGGGCAAATGAAAAGGGTGAATGTTGATTTCCCAAATTGGATGGTAGAAGCGTTAGATAAGC
AAGCCAGACATTTAGGTGTGCCTCGCCAAGCCTTGAGTAAAATGTGGATTGCGGAAAAATTACCGGTTCAATCACCTAGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A107

Antitoxin

Source ID Structure
AlphaFold DB A0A378NDF7

References