Detailed information of TA system TA066973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2335966..2336614
• Replicon: chromosome
• Accession: NZ_CP017492
• Organism: Mannheimia haemolytica strain 38202

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A249A099
• Locus tag: BG569_RS11750
• Protein ID: WP_006251221.1
• Coordinates: 2335966..2336142 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A248ZZL6
• Locus tag: BG569_RS11755
• Protein ID: WP_006251222.1
• Coordinates: 2336198..2336614 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG569_RS11725	2331278..2333773	-	2496	WP_061888644.1	phage tail length tape measure family protein	-
BG569_RS11730	2333860..2334255	-	396	WP_006251217.1	hypothetical protein	-
BG569_RS11735	2334323..2334781	-	459	WP_006251218.1	hypothetical protein	-
BG569_RS11740	2334950..2335183	+	234	WP_020824316.1	hypothetical protein	-
BG569_RS11745	2335198..2335869	-	672	WP_006251220.1	hypothetical protein	-
BG569_RS11750	2335966..2336142	+	177	WP_006251221.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG569_RS11755	2336198..2336614	+	417	WP_006251222.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG569_RS11760	2336654..2337136	-	483	WP_006251223.1	phage tail protein	-
BG569_RS11765	2337140..2337520	-	381	WP_006251224.1	hypothetical protein	-
BG569_RS11770	2337517..2337885	-	369	WP_006251225.1	hypothetical protein	-
BG569_RS11775	2337887..2338231	-	345	WP_006251226.1	hypothetical protein	-
BG569_RS11780	2338231..2338608	-	378	WP_006251227.1	hypothetical protein	-
BG569_RS11785	2338611..2338835	-	225	WP_021265457.1	hypothetical protein	-
BG569_RS11790	2338847..2339833	-	987	WP_062627922.1	encapsulin	-
BG569_RS11795	2339848..2340282	-	435	WP_006251230.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2322292..2370736	48444

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6640.79 Da
• Isoelectric Point: 10.7708

>T66973 WP_006251221.1 NZ_CP017492:2335966-2336142 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Nucleotide

• Length: 177 bp

>T66973 NZ_CP017492:2335966-2336142 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15519.91 Da
• Isoelectric Point: 4.3508

>AT66973 WP_006251222.1 NZ_CP017492:2336198-2336614 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Nucleotide

• Length: 417 bp

>AT66973 NZ_CP017492:2336198-2336614 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Toxin

• AlphaFold DB: A0A249A099

Antitoxin

• AlphaFold DB: A0A248ZZL6