Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1995293..1995933 Replicon chromosome
Accession NZ_CP017492
Organism Mannheimia haemolytica strain 38202

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG569_RS09875 Protein ID WP_006250075.1
Coordinates 1995293..1995475 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NET2
Locus tag BG569_RS09880 Protein ID WP_006250984.1
Coordinates 1995514..1995933 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG569_RS09840 1990864..1991910 - 1047 WP_020824125.1 conserved phage C-terminal domain-containing protein -
BG569_RS09845 1991907..1992746 - 840 WP_006252350.1 Rha family transcriptional regulator -
BG569_RS09850 1992922..1993182 - 261 WP_006252483.1 hypothetical protein -
BG569_RS09855 1993203..1993403 - 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG569_RS09860 1993534..1994217 + 684 WP_006252485.1 helix-turn-helix transcriptional regulator -
BG569_RS09865 1994292..1994672 + 381 WP_006248823.1 hypothetical protein -
BG569_RS09870 1994677..1995162 + 486 WP_006252741.1 hypothetical protein -
BG569_RS09875 1995293..1995475 + 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG569_RS09880 1995514..1995933 + 420 WP_006250984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG569_RS09885 1996000..1996305 - 306 WP_006250985.1 HigA family addiction module antidote protein -
BG569_RS09890 1996314..1996589 - 276 WP_006250986.1 type II toxin-antitoxin system RelE/ParE family toxin -
BG569_RS09895 1996879..1997109 + 231 WP_006250987.1 hypothetical protein -
BG569_RS09900 1997588..1997911 - 324 WP_006250988.1 hypothetical protein -
BG569_RS09905 1998104..1998289 + 186 WP_020824222.1 hypothetical protein -
BG569_RS13350 1998273..1998437 + 165 WP_020824223.1 hypothetical protein -
BG569_RS09910 1998443..1998901 + 459 WP_006253113.1 single-stranded DNA-binding protein -
BG569_RS09915 1998937..1999296 + 360 WP_006250991.1 hypothetical protein -
BG569_RS09920 1999368..2000171 + 804 WP_020824224.1 DUF2303 family protein -
BG569_RS09925 2000223..2000657 + 435 WP_020824225.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1952734..2005742 53008


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T66971 WP_006250075.1 NZ_CP017492:1995293-1995475 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T66971 NZ_CP017492:1995293-1995475 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15957.34 Da        Isoelectric Point: 4.6983

>AT66971 WP_006250984.1 NZ_CP017492:1995514-1995933 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH

Download         Length: 420 bp

>AT66971 NZ_CP017492:1995514-1995933 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATACCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGTAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACGCT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NET2

References