Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-RelB
Location 2350781..2351303 Replicon chromosome
Accession NZ_CP017491
Organism Mannheimia haemolytica strain 38599

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZX63
Locus tag BG598_RS12050 Protein ID WP_006250322.1
Coordinates 2350781..2351050 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A248ZY54
Locus tag BG598_RS12055 Protein ID WP_006250323.1
Coordinates 2351037..2351303 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG598_RS12030 2346101..2346535 - 435 WP_006250318.1 D-tyrosyl-tRNA(Tyr) deacylase -
BG598_RS12035 2346510..2347355 - 846 WP_006250319.1 virulence factor BrkB family protein -
BG598_RS12040 2347357..2348595 - 1239 WP_147008916.1 glutamate-5-semialdehyde dehydrogenase -
BG598_RS12045 2348879..2350762 + 1884 WP_006250321.1 DNA mismatch repair endonuclease MutL -
BG598_RS12050 2350781..2351050 - 270 WP_006250322.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
BG598_RS12055 2351037..2351303 - 267 WP_006250323.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BG598_RS12060 2351743..2352696 + 954 WP_006250325.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
BG598_RS12065 2352765..2353037 + 273 WP_006250326.1 RNA chaperone Hfq -
BG598_RS12070 2353057..2354442 + 1386 WP_006253648.1 GTPase HflX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-84)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10447.99 Da        Isoelectric Point: 6.4732

>T66966 WP_006250322.1 NZ_CP017491:c2351050-2350781 [Mannheimia haemolytica]
MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYCDHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLR
IGSHSHLFG

Download         Length: 270 bp

>T66966 NZ_CP017491:c2351050-2350781 [Mannheimia haemolytica]
ATGCGAGAGATTGAGTATAGTAATGCTTTTAAACGTGACTATAAAAAATGTGCGGTTGAGTCTTTATCCTCTTCATTAAT
TGAGGCGTTGTATTTTCTGATTAATGACAAGCCATTACCTGAGAAATACTGCGATCACGCTTTAACCGGCGAATGGAAAG
GCTTTAGGGATTGCCATATCCAACCTGATTTGGTTCTTATCTATCGAAAAGTGGAAGATGATAAGCTAGAGCTGCTGCGA
ATTGGCAGCCACTCTCATTTATTTGGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9738.10 Da        Isoelectric Point: 4.7954

>AT66966 WP_006250323.1 NZ_CP017491:c2351303-2351037 [Mannheimia haemolytica]
MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATEKRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADL
MEQLNARD

Download         Length: 267 bp

>AT66966 NZ_CP017491:c2351303-2351037 [Mannheimia haemolytica]
ATGGCAAATAGTGCATTAGTAAGCGTAAGAATCAGCCCTGAAATTAAAGAGCAAGCAAGTGAGGTGTTAGCCGGTATCGG
CTTGACTGTCTCTGATGTGATGCGAATGACATTAGCGAAAATCGCAACAGAAAAACGTTTTCAGTTTGATTATCAGCCTA
ATTTTGAGACAGCAGAAGTATTAAGGGCTGTTAAGAATGGCGAGGAAAAGCTACATTCAGCAAATAGTATTGCGGATTTA
ATGGAGCAACTCAATGCGAGAGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZX63

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZY54

References