Detailed information of TA system TA066964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1599356..1599996
• Replicon: chromosome
• Accession: NZ_CP017491
• Organism: Mannheimia haemolytica strain 38599

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A378NFX7
• Locus tag: BG598_RS08170
• Protein ID: WP_006250075.1
• Coordinates: 1599356..1599538 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378NFW9
• Locus tag: BG598_RS08175
• Protein ID: WP_006250074.1
• Coordinates: 1599577..1599996 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG598_RS08125	1594903..1595196	-	294	WP_006247812.1	hypothetical protein	-
BG598_RS08130	1595340..1595729	+	390	WP_015484247.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BG598_RS08135	1595701..1596000	+	300	WP_006247810.1	XRE family transcriptional regulator	-
BG598_RS13695	1596009..1596167	-	159	WP_006247809.1	hypothetical protein	-
BG598_RS13700	1596177..1597244	-	1068	WP_006253284.1	hypothetical protein	-
BG598_RS08150	1597265..1597465	-	201	WP_006248825.1	helix-turn-helix domain-containing protein	-
BG598_RS08155	1597596..1598279	+	684	WP_006248824.1	helix-turn-helix transcriptional regulator	-
BG598_RS08160	1598354..1598734	+	381	WP_006248823.1	hypothetical protein	-
BG598_RS08165	1598739..1599224	+	486	WP_015484250.1	hypothetical protein	-
BG598_RS08170	1599356..1599538	+	183	WP_006250075.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG598_RS08175	1599577..1599996	+	420	WP_006250074.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG598_RS08180	1600332..1600976	+	645	WP_006253274.1	tyrosine-type recombinase/integrase	-
BG598_RS08190	1601114..1602154	+	1041	WP_041447883.1	IS481 family transposase	-
BG598_RS08195	1602221..1603627	-	1407	WP_006253513.1	YdgA family protein	-
BG598_RS08200	1603677..1604483	-	807	WP_006248821.1	tryptophan synthase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1532161..1610664	78503
	-	flank	IS/Tn	-	-	1601114..1602154	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-96)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.03 Da
• Isoelectric Point: 10.3446

>T66964 WP_006250075.1 NZ_CP017491:1599356-1599538 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Nucleotide

• Length: 183 bp

>T66964 NZ_CP017491:1599356-1599538 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15938.30 Da
• Isoelectric Point: 4.6963

>AT66964 WP_006250074.1 NZ_CP017491:1599577-1599996 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Nucleotide

• Length: 420 bp

>AT66964 NZ_CP017491:1599577-1599996 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A378NFX7

Antitoxin

• AlphaFold DB: A0A378NFW9