Detailed information of TA system TA066958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2301113..2301761
• Replicon: chromosome
• Accession: NZ_CP017490
• Organism: Mannheimia haemolytica strain 39107

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A249A099
• Locus tag: BG549_RS11580
• Protein ID: WP_006251221.1
• Coordinates: 2301113..2301289 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A248ZZL6
• Locus tag: BG549_RS11585
• Protein ID: WP_006251222.1
• Coordinates: 2301345..2301761 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG549_RS11555	2296425..2298920	-	2496	WP_061888644.1	phage tail length tape measure family protein	-
BG549_RS11560	2299007..2299402	-	396	WP_006251217.1	hypothetical protein	-
BG549_RS11565	2299470..2299928	-	459	WP_006251218.1	hypothetical protein	-
BG549_RS11570	2300097..2300330	+	234	WP_020824316.1	hypothetical protein	-
BG549_RS11575	2300345..2301016	-	672	WP_006251220.1	hypothetical protein	-
BG549_RS11580	2301113..2301289	+	177	WP_006251221.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG549_RS11585	2301345..2301761	+	417	WP_006251222.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG549_RS11590	2301801..2302283	-	483	WP_006251223.1	phage tail protein	-
BG549_RS11595	2302287..2302667	-	381	WP_006251224.1	hypothetical protein	-
BG549_RS11600	2302664..2303032	-	369	WP_006251225.1	hypothetical protein	-
BG549_RS11605	2303034..2303378	-	345	WP_006251226.1	hypothetical protein	-
BG549_RS11610	2303378..2303755	-	378	WP_006251227.1	hypothetical protein	-
BG549_RS11615	2303758..2304009	-	252	WP_015484272.1	hypothetical protein	-
BG549_RS11620	2304021..2305007	-	987	WP_062627922.1	encapsulin	-
BG549_RS11625	2305022..2305456	-	435	WP_006251230.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2287439..2335847	48408

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6640.79 Da
• Isoelectric Point: 10.7708

>T66958 WP_006251221.1 NZ_CP017490:2301113-2301289 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Nucleotide

• Length: 177 bp

>T66958 NZ_CP017490:2301113-2301289 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15519.91 Da
• Isoelectric Point: 4.3508

>AT66958 WP_006251222.1 NZ_CP017490:2301345-2301761 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Nucleotide

• Length: 417 bp

>AT66958 NZ_CP017490:2301345-2301761 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Toxin

• AlphaFold DB: A0A249A099

Antitoxin

• AlphaFold DB: A0A248ZZL6