Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-RelB
Location 2144402..2144924 Replicon chromosome
Accession NZ_CP017489
Organism Mannheimia haemolytica strain 39309

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZX63
Locus tag BG570_RS10750 Protein ID WP_006250322.1
Coordinates 2144402..2144671 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A248ZY54
Locus tag BG570_RS10755 Protein ID WP_006250323.1
Coordinates 2144658..2144924 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG570_RS10730 2139722..2140156 - 435 WP_006250318.1 D-tyrosyl-tRNA(Tyr) deacylase -
BG570_RS10735 2140131..2140976 - 846 WP_006251985.1 virulence factor BrkB family protein -
BG570_RS10740 2140978..2142216 - 1239 WP_006251984.1 glutamate-5-semialdehyde dehydrogenase -
BG570_RS10745 2142500..2144383 + 1884 WP_006251983.1 DNA mismatch repair endonuclease MutL -
BG570_RS10750 2144402..2144671 - 270 WP_006250322.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
BG570_RS10755 2144658..2144924 - 267 WP_006250323.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BG570_RS10760 2145370..2146323 + 954 WP_006251982.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
BG570_RS10765 2146392..2146664 + 273 WP_006250326.1 RNA chaperone Hfq -
BG570_RS10770 2146684..2148069 + 1386 WP_020824252.1 GTPase HflX -
BG570_RS10780 2148406..2149446 + 1041 WP_020824044.1 IS481 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2148406..2149446 1040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-84)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10447.99 Da        Isoelectric Point: 6.4732

>T66950 WP_006250322.1 NZ_CP017489:c2144671-2144402 [Mannheimia haemolytica]
MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYCDHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLR
IGSHSHLFG

Download         Length: 270 bp

>T66950 NZ_CP017489:c2144671-2144402 [Mannheimia haemolytica]
ATGCGAGAGATTGAGTATAGTAATGCTTTTAAACGTGACTATAAAAAATGTGCGGTTGAGTCTTTATCCTCTTCATTAAT
TGAGGCGTTGTATTTTCTGATTAATGACAAGCCATTACCTGAGAAATACTGCGATCACGCTTTAACCGGCGAATGGAAAG
GTTTTAGGGATTGCCATATCCAACCTGATTTGGTTCTTATCTATCGAAAAGTGGAAGATGATAAGCTAGAGCTGCTGCGA
ATTGGCAGCCACTCTCATTTATTTGGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9738.10 Da        Isoelectric Point: 4.7954

>AT66950 WP_006250323.1 NZ_CP017489:c2144924-2144658 [Mannheimia haemolytica]
MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATEKRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADL
MEQLNARD

Download         Length: 267 bp

>AT66950 NZ_CP017489:c2144924-2144658 [Mannheimia haemolytica]
ATGGCAAATAGTGCATTAGTAAGCGTAAGAATCAGCCCTGAAATTAAAGAGCAAGCAAGTGAGGTGTTAGCCGGTATCGG
CTTGACTGTCTCTGATGTGATGCGAATGACATTAGCGAAAATCGCAACAGAAAAACGTTTTCAGTTTGATTATCAGCCTA
ATTTTGAGACAGCAGAAGTATTAAGGGCTGTTAAGAATGGCGAGGAAAAGCTACATTCAGCAAATAGTATTGCGGATTTA
ATGGAGCAACTCAATGCGAGAGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZX63

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZY54

References