Detailed information of TA system TA066943

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2301103..2301751
• Replicon: chromosome
• Accession: NZ_CP017488
• Organism: Mannheimia haemolytica strain 39373

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A249A099
• Locus tag: BG550_RS11590
• Protein ID: WP_006251221.1
• Coordinates: 2301103..2301279 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A248ZZL6
• Locus tag: BG550_RS11595
• Protein ID: WP_006251222.1
• Coordinates: 2301335..2301751 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG550_RS11565	2296415..2298910	-	2496	WP_061888644.1	phage tail length tape measure family protein	-
BG550_RS11570	2298997..2299392	-	396	WP_006251217.1	hypothetical protein	-
BG550_RS11575	2299460..2299918	-	459	WP_006251218.1	hypothetical protein	-
BG550_RS11580	2300051..2300320	+	270	WP_006251219.1	hypothetical protein	-
BG550_RS11585	2300335..2301006	-	672	WP_006251220.1	hypothetical protein	-
BG550_RS11590	2301103..2301279	+	177	WP_006251221.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG550_RS11595	2301335..2301751	+	417	WP_006251222.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG550_RS11600	2301791..2302273	-	483	WP_006251223.1	phage tail protein	-
BG550_RS11605	2302277..2302657	-	381	WP_006251224.1	hypothetical protein	-
BG550_RS11610	2302654..2303022	-	369	WP_006251225.1	hypothetical protein	-
BG550_RS11615	2303024..2303368	-	345	WP_006251226.1	hypothetical protein	-
BG550_RS11620	2303368..2303745	-	378	WP_006251227.1	hypothetical protein	-
BG550_RS11625	2303748..2303999	-	252	WP_015484272.1	hypothetical protein	-
BG550_RS11630	2304011..2304997	-	987	WP_062627922.1	encapsulin	-
BG550_RS11635	2305012..2305446	-	435	WP_006251230.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2287429..2335837	48408

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6640.79 Da
• Isoelectric Point: 10.7708

>T66943 WP_006251221.1 NZ_CP017488:2301103-2301279 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Nucleotide

• Length: 177 bp

>T66943 NZ_CP017488:2301103-2301279 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15519.91 Da
• Isoelectric Point: 4.3508

>AT66943 WP_006251222.1 NZ_CP017488:2301335-2301751 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Nucleotide

• Length: 417 bp

>AT66943 NZ_CP017488:2301335-2301751 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Toxin

• AlphaFold DB: A0A249A099

Antitoxin

• AlphaFold DB: A0A248ZZL6