66921

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-RelB
Location 2144408..2144930 Replicon chromosome
Accession NZ_CP017485
Organism Mannheimia haemolytica strain 39409

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZX63
Locus tag BG571_RS10760 Protein ID WP_006250322.1
Coordinates 2144408..2144677 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A248ZY54
Locus tag BG571_RS10765 Protein ID WP_006250323.1
Coordinates 2144664..2144930 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG571_RS10740 2139728..2140162 - 435 WP_006250318.1 D-tyrosyl-tRNA(Tyr) deacylase -
BG571_RS10745 2140137..2140982 - 846 WP_006251985.1 virulence factor BrkB family protein -
BG571_RS10750 2140984..2142222 - 1239 WP_006251984.1 glutamate-5-semialdehyde dehydrogenase -
BG571_RS10755 2142506..2144389 + 1884 WP_006251983.1 DNA mismatch repair endonuclease MutL -
BG571_RS10760 2144408..2144677 - 270 WP_006250322.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
BG571_RS10765 2144664..2144930 - 267 WP_006250323.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BG571_RS10770 2145376..2146329 + 954 WP_006251982.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
BG571_RS10775 2146398..2146670 + 273 WP_006250326.1 RNA chaperone Hfq -
BG571_RS10780 2146690..2148075 + 1386 WP_020824252.1 GTPase HflX -
BG571_RS10790 2148412..2149452 + 1041 WP_020824044.1 IS481 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2148412..2149452 1040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-84)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10447.99 Da        Isoelectric Point: 6.4732

>T66921 WP_006250322.1 NZ_CP017485:c2144677-2144408 [Mannheimia haemolytica]
MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYCDHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLR
IGSHSHLFG

Download         Length: 270 bp

>T66921 NZ_CP017485:c2144677-2144408 [Mannheimia haemolytica]
ATGCGAGAGATTGAGTATAGTAATGCTTTTAAACGTGACTATAAAAAATGTGCGGTTGAGTCTTTATCCTCTTCATTAAT
TGAGGCGTTGTATTTTCTGATTAATGACAAGCCATTACCTGAGAAATACTGCGATCACGCTTTAACCGGCGAATGGAAAG
GTTTTAGGGATTGCCATATCCAACCTGATTTGGTTCTTATCTATCGAAAAGTGGAAGATGATAAGCTAGAGCTGCTGCGA
ATTGGCAGCCACTCTCATTTATTTGGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9738.10 Da        Isoelectric Point: 4.7954

>AT66921 WP_006250323.1 NZ_CP017485:c2144930-2144664 [Mannheimia haemolytica]
MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATEKRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADL
MEQLNARD

Download         Length: 267 bp

>AT66921 NZ_CP017485:c2144930-2144664 [Mannheimia haemolytica]
ATGGCAAATAGTGCATTAGTAAGCGTAAGAATCAGCCCTGAAATTAAAGAGCAAGCAAGTGAGGTGTTAGCCGGTATCGG
CTTGACTGTCTCTGATGTGATGCGAATGACATTAGCGAAAATCGCAACAGAAAAACGTTTTCAGTTTGATTATCAGCCTA
ATTTTGAGACAGCAGAAGTATTAAGGGCTGTTAAGAATGGCGAGGAAAAGCTACATTCAGCAAATAGTATTGCGGATTTA
ATGGAGCAACTCAATGCGAGAGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZX63

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZY54

References