Detailed information of TA system TA066902

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4218382..4218974
• Replicon: chromosome
• Accession: NZ_CP017482
• Organism: Pectobacterium polaris strain NIBIO1392

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: BJK05_RS19160
• Protein ID: WP_095702005.1
• Coordinates: 4218382..4218636 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: BJK05_RS19165
• Protein ID: WP_095701362.1
• Coordinates: 4218633..4218974 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJK05_RS19145	4214777..4215328	+	552	WP_095701360.1	polymer-forming cytoskeletal protein	-
BJK05_RS19150	4215365..4218085	+	2721	WP_095701361.1	CRISPR-associated helicase/endonuclease Cas3	-
BJK05_RS19160	4218382..4218636	+	255	WP_095702005.1	type II toxin-antitoxin system HicA family toxin	Toxin
BJK05_RS19165	4218633..4218974	+	342	WP_095701362.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BJK05_RS19170	4219605..4221164	+	1560	WP_095701363.1	type I-E CRISPR-associated protein Cse1/CasA	-
BJK05_RS19175	4221157..4221804	+	648	WP_095701364.1	type I-E CRISPR-associated protein Cse2/CasB	-
BJK05_RS19180	4221808..4222872	+	1065	WP_095701365.1	type I-E CRISPR-associated protein Cas7/Cse4/CasC	-
BJK05_RS19185	4222882..4223616	+	735	WP_095701366.1	type I-E CRISPR-associated protein Cas5/CasD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (55-100)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9540.03 Da
• Isoelectric Point: 11.1073

>T66902 WP_095702005.1 NZ_CP017482:4218382-4218636 [Pectobacterium polaris]
MNKRHQKTLSDLFSRPVNGSIKWSDIESLFVALGAEIHEREGSRIAVLLKGEKKIFHRPHPRPTTDKGAVNSIRVWLESL
GVRP

Nucleotide

• Length: 255 bp

>T66902 NZ_CP017482:4218382-4218636 [Pectobacterium polaris]
ATGAATAAAAGACACCAAAAAACGCTATCAGATCTGTTTTCTCGTCCAGTCAATGGTTCAATAAAATGGAGCGACATAGA
ATCTCTCTTTGTTGCTCTAGGGGCTGAAATTCATGAAAGGGAAGGTTCGAGGATTGCGGTATTGTTGAAAGGTGAGAAAA
AAATTTTTCATCGACCACATCCTAGACCGACTACTGACAAAGGAGCGGTTAACTCCATTCGGGTATGGTTAGAAAGTTTA
GGAGTAAGACCATGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12384.98 Da
• Isoelectric Point: 4.4547

>AT66902 WP_095701362.1 NZ_CP017482:4218633-4218974 [Pectobacterium polaris]
MINTLLIDGHTAVIAFDSEIEMFRGEFIGLNGGADFYAYSVDELKKEGATSLAIFLDECRRDGIEPYKSYSGKVTTRLTP
ERHAALAVTAQATGVSINELLNEGVDLVLERRS

Nucleotide

• Length: 342 bp

>AT66902 NZ_CP017482:4218633-4218974 [Pectobacterium polaris]
ATGATTAACACACTTCTTATCGACGGCCACACTGCCGTTATTGCTTTTGACTCGGAAATTGAAATGTTCCGGGGAGAATT
CATTGGCTTGAATGGCGGCGCTGATTTTTATGCTTACAGCGTTGATGAGCTGAAAAAAGAAGGGGCAACCTCTCTAGCTA
TTTTTCTTGATGAATGCCGCCGTGATGGTATTGAGCCCTATAAATCCTACAGTGGAAAAGTCACAACACGTTTGACGCCA
GAACGTCATGCTGCGCTGGCGGTAACGGCGCAGGCAACTGGCGTTTCCATTAACGAATTGTTAAACGAAGGCGTCGATCT
GGTCCTTGAACGACGTTCTTAA