Detailed information of TA system TA066869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2506900..2507579
• Replicon: chromosome
• Accession: NZ_CP017476
• Organism: Hydrogenophaga crassostreae strain LPB0072

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A162P622
• Locus tag: LPB072_RS11585
• Protein ID: WP_066089765.1
• Coordinates: 2507154..2507579 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A167HSI0
• Locus tag: LPB072_RS11580
• Protein ID: WP_066089762.1
• Coordinates: 2506900..2507157 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPB072_RS11550	2502724..2503575	-	852	WP_066089745.1	hypothetical protein	-
LPB072_RS11555	2503589..2504344	-	756	WP_066089748.1	DsbC family protein	-
LPB072_RS11560	2504382..2504894	-	513	WP_082876888.1	PH domain-containing protein	-
LPB072_RS11565	2504916..2505218	-	303	WP_066089751.1	hypothetical protein	-
LPB072_RS11570	2505413..2505847	-	435	WP_066089754.1	hypothetical protein	-
LPB072_RS11575	2506028..2506810	-	783	WP_066089759.1	SprT-like domain-containing protein	-
LPB072_RS11580	2506900..2507157	+	258	WP_066089762.1	plasmid stability protein stbC	Antitoxin
LPB072_RS11585	2507154..2507579	+	426	WP_066089765.1	type II toxin-antitoxin system VapC family toxin	Toxin
LPB072_RS11590	2507586..2507807	+	222	WP_066089768.1	hypothetical protein	-
LPB072_RS23445	2507884..2508102	+	219	WP_157559295.1	hypothetical protein	-
LPB072_RS11595	2508120..2509709	-	1590	WP_066089770.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
LPB072_RS11600	2509767..2510159	-	393	WP_157559296.1	hypothetical protein	-
LPB072_RS11605	2510156..2511538	-	1383	WP_082876889.1	integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2457377..2521414	64037

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15363.75 Da
• Isoelectric Point: 5.1972

>T66869 WP_066089765.1 NZ_CP017476:2507154-2507579 [Hydrogenophaga crassostreae]
MILLDTNVISELWKVEPNPQVFAWIDAQTVETLYLSAVTVAELRFGLATMPGSKRRRIFQDRLEEVVLPTFAERVLPFDL
ATSHAYAGLMAKARSEGKAISREDGYIAATAIAGGLIVATRDTSPFAAAGCKVINPWEPIG

Nucleotide

• Length: 426 bp

>T66869 NZ_CP017476:2507154-2507579 [Hydrogenophaga crassostreae]
ATGATCCTGCTCGACACCAACGTAATCTCCGAGCTATGGAAGGTCGAGCCGAACCCGCAGGTGTTCGCCTGGATTGATGC
TCAGACCGTAGAAACGCTCTACCTATCGGCCGTCACGGTTGCGGAGCTGCGATTTGGCTTGGCAACAATGCCGGGAAGCA
AGCGGCGTAGGATTTTTCAAGACAGATTGGAGGAGGTGGTGCTGCCTACCTTTGCGGAGCGCGTACTGCCCTTCGACCTG
GCCACTTCACATGCCTATGCAGGCTTGATGGCCAAGGCGAGATCCGAAGGCAAGGCCATAAGCAGGGAAGACGGCTACAT
CGCGGCGACGGCCATTGCCGGTGGCCTCATCGTCGCAACAAGAGACACCAGCCCGTTTGCGGCTGCAGGGTGCAAAGTCA
TCAACCCATGGGAGCCGATTGGCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9380.64 Da
• Isoelectric Point: 9.1614

>AT66869 WP_066089762.1 NZ_CP017476:2506900-2507157 [Hydrogenophaga crassostreae]
MANVTVRNLPDAVHRALRVRAATHGRSTEAEIRNILEEAARPTQRIRLGSLLVSIAREAGGITEAEAAHFDQLRDKNAAE
PMRIE

Nucleotide

• Length: 258 bp

>AT66869 NZ_CP017476:2506900-2507157 [Hydrogenophaga crassostreae]
ATGGCAAACGTCACTGTCCGAAACCTGCCAGACGCAGTGCATAGGGCCTTGCGCGTGCGCGCGGCTACGCACGGCCGAAG
CACAGAGGCTGAAATCCGAAACATCTTGGAGGAGGCCGCCAGGCCAACGCAGAGAATCAGGCTTGGATCATTGTTGGTGT
CCATCGCCCGCGAGGCTGGCGGCATAACCGAAGCTGAAGCGGCGCATTTTGACCAGCTCCGCGACAAGAACGCTGCTGAA
CCAATGAGGATCGAATGA

Structures

Toxin

• AlphaFold DB: A0A162P622

Antitoxin

• AlphaFold DB: A0A167HSI0