Detailed information of TA system TA066867

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4172091..4172879
• Replicon: chromosome
• Accession: NZ_CP017475
• Organism: Enterobacter cloacae strain M12X01451

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: GRI03_RS20670
• Protein ID: WP_095908291.1
• Coordinates: 4172091..4172468 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: GRI03_RS20675
• Protein ID: WP_095908292.1
• Coordinates: 4172520..4172879 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GRI03_RS20635	4167298..4167807	-	510	WP_015386351.1	phage regulatory CII family protein	-
GRI03_RS20640	4167842..4168078	-	237	WP_023206292.1	hypothetical protein	-
GRI03_RS20645	4168166..4168816	+	651	WP_095908289.1	phage repressor protein CI	-
GRI03_RS20650	4168842..4169387	+	546	WP_023206294.1	hypothetical protein	-
GRI03_RS20655	4169393..4170406	+	1014	WP_023206295.1	tyrosine-type recombinase/integrase	-
GRI03_RS20660	4170668..4171513	-	846	WP_095908290.1	DUF4942 domain-containing protein	-
GRI03_RS20665	4171603..4172094	-	492	Protein_3870	hypothetical protein	-
GRI03_RS20670	4172091..4172468	-	378	WP_095908291.1	TA system toxin CbtA family protein	Toxin
GRI03_RS20675	4172520..4172879	-	360	WP_095908292.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GRI03_RS20680	4172975..4173196	-	222	WP_095908293.1	DUF987 domain-containing protein	-
GRI03_RS20685	4173208..4173687	-	480	WP_095908294.1	DNA repair protein RadC	-
GRI03_RS20690	4173699..4174160	-	462	WP_095908295.1	antirestriction protein	-
GRI03_RS20695	4174171..4174419	-	249	WP_095908296.1	hypothetical protein	-
GRI03_RS20700	4174419..4175237	-	819	WP_095908297.1	DUF945 domain-containing protein	-
GRI03_RS20705	4175363..4175836	-	474	WP_095908298.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4126377..4183105	56728

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (4-112)

Antitoxin

• Antitoxin CbeA (16-114)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13910.84 Da
• Isoelectric Point: 9.3401

>T66867 WP_095908291.1 NZ_CP017475:c4172468-4172091 [Enterobacter cloacae]
MYIMSASYRRTAPSRPSPVTVWQTLLTHLLDRHYGLTLNDTPFGNDGVIQEHIEAGISLCYAVNFIVEKYDLVRTDRSGF
SAETSSPFLSSIDILRARKATGLMARTGYRAVTDITTGKYSRMAG

Nucleotide

• Length: 378 bp

>T66867 NZ_CP017475:c4172468-4172091 [Enterobacter cloacae]
ATGTACATCATGTCTGCATCTTACCGGCGGACGGCACCGTCACGTCCGTCGCCGGTGACCGTCTGGCAAACCCTGCTGAC
GCATCTGCTCGACCGTCATTACGGTCTCACGCTCAACGACACCCCGTTTGGTAACGATGGTGTTATTCAGGAACACATTG
AGGCCGGTATCTCCCTGTGTTATGCCGTCAATTTTATTGTCGAAAAATACGACCTTGTGCGCACTGACCGCAGCGGCTTC
AGCGCTGAAACCTCATCACCGTTTCTCAGCAGTATCGATATTCTGCGGGCGCGTAAGGCGACCGGGCTGATGGCCCGCAC
GGGCTACAGAGCGGTGACCGACATAACCACCGGCAAATACAGCAGGATGGCAGGATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13335.16 Da
• Isoelectric Point: 6.2066

>AT66867 WP_095908292.1 NZ_CP017475:c4172879-4172520 [Enterobacter cloacae]
MPDIDHTTNDSMDAPRWGLQRDIIPCFGARLVQEGNRLHYLADRASITGTFSDADLRHLDQAFSLLMKQMELMLTTGELT
PRHPHRVTLYAKGLTCEADSLGSHGYIYIAIYPTLSVTS

Nucleotide

• Length: 360 bp

>AT66867 NZ_CP017475:c4172879-4172520 [Enterobacter cloacae]
ATGCCAGACATCGACCACACCACAAATGACAGTATGGACGCCCCGCGCTGGGGTCTGCAGCGCGATATCATCCCGTGCTT
TGGCGCACGGCTGGTGCAGGAAGGCAACCGCCTTCACTACCTGGCTGACCGCGCCAGTATTACCGGGACCTTCAGTGACG
CGGATTTACGCCATCTGGACCAGGCGTTTTCGCTGCTGATGAAGCAGATGGAACTGATGCTCACTACCGGAGAACTCACT
CCCCGCCACCCGCACCGGGTCACGTTGTATGCAAAGGGACTGACCTGCGAGGCCGATTCACTCGGATCGCATGGCTATAT
CTATATAGCGATTTATCCGACACTATCAGTAACGTCATAA