Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1115620..1116240 Replicon chromosome
Accession NZ_CP017475
Organism Enterobacter cloacae strain M12X01451

Toxin (Protein)


Gene name Hha Uniprot ID A0A5E1A1U8
Locus tag GRI03_RS06070 Protein ID WP_013095889.1
Coordinates 1115620..1115838 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag GRI03_RS06075 Protein ID WP_008499288.1
Coordinates 1115866..1116240 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRI03_RS06040 1111632..1111892 + 261 WP_006176933.1 type B 50S ribosomal protein L31 -
GRI03_RS06045 1111895..1112035 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
GRI03_RS06050 1112032..1112742 - 711 WP_023620096.1 GNAT family N-acetyltransferase -
GRI03_RS06055 1112844..1114304 + 1461 WP_095907421.1 PLP-dependent aminotransferase family protein -
GRI03_RS06060 1114276..1114743 - 468 WP_013095887.1 membrane protein -
GRI03_RS06065 1114859..1115410 - 552 WP_013095888.1 maltose O-acetyltransferase -
GRI03_RS06070 1115620..1115838 - 219 WP_013095889.1 hemolysin expression modulator Hha Toxin
GRI03_RS06075 1115866..1116240 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
GRI03_RS06080 1116753..1119899 - 3147 WP_013095890.1 multidrug efflux RND transporter permease subunit -
GRI03_RS06085 1119922..1121115 - 1194 WP_023620093.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8625.98 Da        Isoelectric Point: 8.9008

>T66854 WP_013095889.1 NZ_CP017475:c1115838-1115620 [Enterobacter cloacae]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELTVFYSAADHRLAELTMNKLYDKIPPSVWKFVR

Download         Length: 219 bp

>T66854 NZ_CP017475:c1115838-1115620 [Enterobacter cloacae]
ATGTCTGATAAACCACTCACAAAAACCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACACTCGAACGCGT
GATTGAAAAAAATAAATACGAGCTTTCTGATAATGAACTGACCGTATTTTATTCAGCCGCTGACCATCGCCTTGCCGAGC
TGACCATGAATAAACTGTATGACAAAATCCCGCCTTCCGTGTGGAAATTTGTCCGTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT66854 WP_008499288.1 NZ_CP017475:c1116240-1115866 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT66854 NZ_CP017475:c1116240-1115866 [Enterobacter cloacae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAATCATGGCTGGGTCAACGATCCAACATCGGCCATCAATTTGCAATTGAATGAGTTGATAGAGC
ATATCGCGACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAACTGATTGAGCAAATTGACGAATACCTG
GATGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCGCAGGATTTGCAGAAATGGCGTAAATCTGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGCCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1A1U8

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References