Detailed information of TA system TA066853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): KacT-ataR/DUF1778(antitoxin)
• Location: 584558..585352
• Replicon: chromosome
• Accession: NZ_CP017475
• Organism: Enterobacter cloacae strain M12X01451

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: GRI03_RS03660
• Protein ID: WP_095907305.1
• Coordinates: 584558..585079 (-)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A5E1AP76
• Locus tag: GRI03_RS03665
• Protein ID: WP_013095428.1
• Coordinates: 585083..585352 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GRI03_RS03635	579885..580046	+	162	WP_020687816.1	DUF1127 domain-containing protein	-
GRI03_RS03640	580133..581521	-	1389	WP_047023206.1	PLP-dependent aminotransferase family protein	-
GRI03_RS03645	581624..582103	+	480	WP_013095424.1	carboxymuconolactone decarboxylase family protein	-
GRI03_RS03650	582090..582992	-	903	WP_023620661.1	LysR family transcriptional regulator	-
GRI03_RS03655	583093..584508	+	1416	WP_095907304.1	aldehyde dehydrogenase family protein	-
GRI03_RS03660	584558..585079	-	522	WP_095907305.1	GNAT family N-acetyltransferase	Toxin
GRI03_RS03665	585083..585352	-	270	WP_013095428.1	DUF1778 domain-containing protein	Antitoxin
GRI03_RS03670	585567..585890	+	324	WP_023620659.1	antibiotic biosynthesis monooxygenase	-
GRI03_RS03675	585883..586281	+	399	WP_095907306.1	amino acid-binding protein	-
GRI03_RS03680	586253..586948	+	696	WP_038418621.1	winged helix-turn-helix domain-containing protein	-
GRI03_RS03685	587611..588081	+	471	WP_095907307.1	MarR family transcriptional regulator	-
GRI03_RS03690	588078..589145	+	1068	WP_095907308.1	HlyD family secretion protein	-
GRI03_RS03695	589135..590211	+	1077	WP_013095434.1	DUF2955 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-157)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-88)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19376.32 Da
• Isoelectric Point: 9.2434

>T66853 WP_095907305.1 NZ_CP017475:c585079-584558 [Enterobacter cloacae]
VNNMKIGIFSDDVEYDLSHFDCGEESLNTFLTAHLKRQHRGKFLRGYVLVASGEKPRVLGYYTLSGSCFEKAYLPSKTQQ
KRVPYKNVPSVTLGRLAIDKRLQGQGLGELLVTHALKTVYLASFAVGIHGIFVEALNDSAKNFYLKLGFIALQAENENTL
FLPTKTIERLFAD

Nucleotide

• Length: 522 bp

>T66853 NZ_CP017475:c585079-584558 [Enterobacter cloacae]
GTGAATAACATGAAAATAGGGATTTTTTCAGATGACGTTGAGTATGATCTAAGCCATTTCGATTGCGGAGAAGAGAGCCT
AAACACCTTTCTGACGGCGCACCTGAAACGCCAGCATCGTGGGAAATTTTTGCGGGGTTACGTTCTTGTCGCATCCGGGG
AAAAGCCGCGGGTGTTGGGTTATTACACGCTGTCCGGGAGCTGCTTTGAAAAGGCGTACCTTCCTTCTAAAACCCAGCAG
AAGCGAGTGCCTTACAAAAATGTTCCCAGCGTCACGCTAGGCCGCCTGGCGATAGATAAACGCTTACAGGGGCAAGGATT
GGGGGAGTTGCTGGTCACCCATGCTCTGAAAACGGTCTACCTGGCGTCTTTTGCCGTGGGTATCCACGGGATATTCGTAG
AAGCGCTGAACGACAGCGCCAAAAACTTTTACCTTAAACTGGGGTTTATCGCTCTTCAGGCAGAGAATGAAAATACTCTC
TTCCTGCCGACTAAAACCATCGAAAGATTGTTTGCAGATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10177.54 Da
• Isoelectric Point: 5.8652

>AT66853 WP_013095428.1 NZ_CP017475:c585352-585083 [Enterobacter cloacae]
MSALKKQRIDLRLTEDDKSLIEEAAAMTNQSISQFMVSTASERAAEVIEQHRRLILNEASWNQVMDAISNPPTPNERLKR
AARRLQDLE

Nucleotide

• Length: 270 bp

>AT66853 NZ_CP017475:c585352-585083 [Enterobacter cloacae]
ATGTCAGCACTAAAGAAACAGCGCATCGATCTACGCTTAACCGAAGACGACAAAAGCCTGATCGAAGAAGCAGCGGCAAT
GACGAACCAGTCAATTTCACAATTCATGGTAAGTACGGCCTCCGAGCGCGCAGCAGAAGTGATTGAACAGCACCGCCGCC
TGATCCTCAACGAGGCATCCTGGAACCAGGTGATGGATGCGATCAGTAACCCGCCAACGCCAAATGAACGACTGAAGCGA
GCAGCCAGGCGTCTCCAGGATCTGGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A5E1AP76