Detailed information of TA system TA066852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 502549..503065
• Replicon: chromosome
• Accession: NZ_CP017475
• Organism: Enterobacter cloacae strain M12X01451

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GRI03_RS03310
• Protein ID: WP_040020450.1
• Coordinates: 502781..503065 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V3EXX3
• Locus tag: GRI03_RS03305
• Protein ID: WP_008501400.1
• Coordinates: 502549..502791 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GRI03_RS03290	498544..499284	+	741	WP_062934813.1	KDGP aldolase family protein	-
GRI03_RS03295	499405..500541	+	1137	WP_095907274.1	lactonase family protein	-
GRI03_RS03300	500561..502471	+	1911	WP_048971365.1	BglG family transcription antiterminator	-
GRI03_RS03305	502549..502791	+	243	WP_008501400.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
GRI03_RS03310	502781..503065	+	285	WP_040020450.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GRI03_RS03315	503069..503533	-	465	WP_045902458.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
GRI03_RS03320	503669..505807	-	2139	WP_058660602.1	anaerobic ribonucleoside-triphosphate reductase	-
GRI03_RS03325	506181..507824	-	1644	WP_095907275.1	alpha,alpha-phosphotrehalase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10958.82 Da
• Isoelectric Point: 10.4610

>T66852 WP_040020450.1 NZ_CP017475:502781-503065 [Enterobacter cloacae]
MTYELEFDPRALKEWSKLGETVKKQFRKKLAGVLVNPRIESARLHNLPDCYKIKLRSQGYRLVYQVQDNVVTVVVIAIGK
REKSAVYHDANKRL

Nucleotide

• Length: 285 bp

>T66852 NZ_CP017475:502781-503065 [Enterobacter cloacae]
ATGACTTATGAACTGGAATTTGACCCGAGGGCATTAAAGGAGTGGAGCAAGCTGGGGGAGACTGTAAAAAAGCAGTTCAG
GAAGAAACTTGCCGGGGTGTTGGTAAACCCCCGGATCGAATCAGCCCGTCTGCATAATCTGCCTGATTGCTACAAAATAA
AACTCAGATCCCAGGGTTACCGTTTGGTCTATCAGGTTCAGGACAACGTGGTAACGGTAGTTGTCATCGCCATTGGGAAA
AGAGAGAAATCAGCCGTATATCACGATGCTAACAAACGGCTATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8972.37 Da
• Isoelectric Point: 5.0651

>AT66852 WP_008501400.1 NZ_CP017475:502549-502791 [Enterobacter cloacae]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTISDSEDALIQTVRERLANPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT66852 NZ_CP017475:502549-502791 [Enterobacter cloacae]
ATGGCTACGCTTAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTGGAAAAACTCAATATCACCCC
AACAGAAGCCGTGCGACTCCTTTTTCAGTACGTTGCGGAGACGGGGCGCATGCCGGTGAAAACGGTCACGATTAGCGACA
GCGAAGATGCGCTGATACAGACAGTCCGGGAGCGACTGGCTAATCCGCAAAAGGGAATTAAGGTTAGTCTGGATGACTTA
TGA

Structures

Antitoxin

• AlphaFold DB: V3EXX3