Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 172746..173350 Replicon chromosome
Accession NZ_CP017475
Organism Enterobacter cloacae strain M12X01451

Toxin (Protein)


Gene name hicA Uniprot ID A0A421IJD5
Locus tag GRI03_RS01745 Protein ID WP_071843252.1
Coordinates 172746..172931 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7Z1F6W0
Locus tag GRI03_RS01750 Protein ID WP_013094943.1
Coordinates 172946..173350 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRI03_RS01730 169177..170715 + 1539 WP_095907210.1 aldehyde dehydrogenase -
GRI03_RS01735 170712..171677 - 966 WP_061771396.1 LysR family transcriptional regulator -
GRI03_RS01740 171795..172535 + 741 WP_095907211.1 MipA/OmpV family protein -
GRI03_RS01745 172746..172931 + 186 WP_071843252.1 type II toxin-antitoxin system HicA family toxin Toxin
GRI03_RS01750 172946..173350 + 405 WP_013094943.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GRI03_RS01755 173404..175359 - 1956 WP_095907212.1 glycoside hydrolase family 127 protein -
GRI03_RS01760 175370..176770 - 1401 WP_020686524.1 MFS transporter -
GRI03_RS01765 176995..177813 + 819 WP_095907213.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6859.08 Da        Isoelectric Point: 11.7891

>T66851 WP_071843252.1 NZ_CP017475:172746-172931 [Enterobacter cloacae]
VKSADVITVLVRHGWKCVRTKGSHHQFRHPVHAGLVTVPHPKKDIKPGTLAQIWRQAGIKH

Download         Length: 186 bp

>T66851 NZ_CP017475:172746-172931 [Enterobacter cloacae]
GTGAAAAGTGCGGATGTCATTACTGTTCTGGTCAGGCATGGCTGGAAATGTGTAAGAACGAAAGGAAGCCATCATCAGTT
TCGCCACCCAGTACATGCGGGGTTGGTGACCGTTCCACATCCCAAAAAGGACATAAAACCCGGTACCCTCGCGCAAATCT
GGCGGCAGGCGGGAATCAAACACTGA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14915.74 Da        Isoelectric Point: 4.3036

>AT66851 WP_013094943.1 NZ_CP017475:172946-173350 [Enterobacter cloacae]
MFYPAYIHSDPDGSASGFFPDVPGCFFAGNSLDDAFQDARDALTAHFEALFEMDEELPLPGNVEAHLEATPGDFIGGQWL
LVDINMKQFDGKAERINITLPRRLLVKIDSFVNEHPQFSNRSAFLAEAARRVLP

Download         Length: 405 bp

>AT66851 NZ_CP017475:172946-173350 [Enterobacter cloacae]
ATGTTTTATCCGGCTTACATTCATTCTGATCCCGACGGTAGCGCCAGCGGTTTTTTTCCTGACGTTCCTGGTTGTTTTTT
TGCCGGCAATTCTCTGGATGATGCTTTTCAGGATGCACGTGATGCACTGACAGCTCACTTCGAAGCGCTATTTGAAATGG
ATGAAGAGTTACCTCTCCCTGGCAATGTTGAAGCACATCTTGAAGCCACACCGGGTGATTTTATCGGCGGACAATGGTTG
CTTGTAGATATCAATATGAAGCAATTCGACGGTAAGGCCGAGCGTATCAATATCACCCTGCCTCGTCGATTGCTGGTGAA
AATTGATTCTTTTGTGAATGAGCATCCGCAGTTTAGTAACCGAAGCGCTTTTCTTGCTGAGGCAGCGCGTCGCGTGCTGC
CTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A421IJD5

Antitoxin

Source ID Structure
AlphaFold DB A0A7Z1F6W0

References