Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 154230..154873 Replicon plasmid pM12X01451
Accession NZ_CP017473
Organism Enterobacter cloacae strain M12X01451

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag GRI03_RS00830 Protein ID WP_095907152.1
Coordinates 154230..154646 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID -
Locus tag GRI03_RS00835 Protein ID WP_095907153.1
Coordinates 154643..154873 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRI03_RS00795 150022..150492 - 471 WP_095907148.1 hypothetical protein -
GRI03_RS00800 150576..151148 - 573 WP_095907149.1 FAD-dependent oxidoreductase -
GRI03_RS00805 151309..152163 + 855 WP_095907150.1 AraC family transcriptional regulator -
GRI03_RS00810 152397..152495 - 99 Protein_161 Rpn family recombination-promoting nuclease/putative transposase -
GRI03_RS00815 152614..153468 - 855 WP_095907060.1 IS3 family transposase -
GRI03_RS00820 153465..153749 - 285 WP_095907061.1 transposase -
GRI03_RS00825 153832..154071 - 240 WP_157735648.1 SdpI family protein -
GRI03_RS00830 154230..154646 - 417 WP_095907152.1 type II toxin-antitoxin system VapC family toxin Toxin
GRI03_RS00835 154643..154873 - 231 WP_095907153.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GRI03_RS00840 155243..157293 + 2051 Protein_167 hypothetical protein -
GRI03_RS00845 157358..158140 - 783 Protein_168 IS21-like element ISSen3 family helper ATPase IstB -
GRI03_RS00850 158137..159159 - 1023 WP_095907091.1 IS21 family transposase -
GRI03_RS00855 159246..159843 + 598 Protein_170 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..169226 169226
- inside IScluster/Tn - - 152614..159623 7009


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15135.50 Da        Isoelectric Point: 7.1083

>T66848 WP_095907152.1 NZ_CP017473:c154646-154230 [Enterobacter cloacae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVISAITYSEMRFGATGPKASPRHVQLVDEFCSRLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAVAASAVLVTNNTREFERVPGLVLEDWAK

Download         Length: 417 bp

>T66848 NZ_CP017473:c154646-154230 [Enterobacter cloacae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCATTCATCATGCGCGAGCAGCCGGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTGATCTCGGCTATCACTTACTCCGAAATGCGCTTCGGCGCCACCGGGC
CGAAGGCCTCGCCCCGTCACGTGCAGCTGGTTGATGAATTCTGCAGCCGCCTCGATGCCATCCTGCCCTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTTGCGCTGCGCCTTGCCGGCACGCCAATCGGCCCGAACGACACGGCGATAGC
CGGGCATGCTGTCGCCGCCAGTGCCGTACTGGTGACGAATAATACGCGGGAGTTTGAGCGGGTGCCTGGGTTAGTACTTG
AAGACTGGGCGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8696.77 Da        Isoelectric Point: 4.3654

>AT66848 WP_095907153.1 NZ_CP017473:c154873-154643 [Enterobacter cloacae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFASLEKADPDFMAEREDVVTDEGRFDL

Download         Length: 231 bp

>AT66848 NZ_CP017473:c154873-154643 [Enterobacter cloacae]
ATGAGGACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAACTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCTGTCCGGCCGACCTGGGGCTCATTCGCCAGCCTGG
AAAAAGCCGATCCGGATTTTATGGCGGAGCGTGAGGACGTTGTCACCGACGAAGGACGATTTGACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References