Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 27350..27875 Replicon plasmid pM12X01451
Accession NZ_CP017473
Organism Enterobacter cloacae strain M12X01451

Toxin (Protein)


Gene name ccdB Uniprot ID -
Locus tag GRI03_RS00125 Protein ID WP_095907065.1
Coordinates 27350..27655 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID -
Locus tag GRI03_RS00130 Protein ID WP_095907066.1
Coordinates 27657..27875 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRI03_RS00110 24686..25915 - 1230 WP_095907064.1 autotransporter strand-loop-strand O-heptosyltransferase -
GRI03_RS00115 26656..27152 - 497 Protein_22 tyrosine-type recombinase/integrase -
GRI03_RS00120 27128..27348 - 221 Protein_23 cytoplasmic protein -
GRI03_RS00125 27350..27655 - 306 WP_095907065.1 type II toxin-antitoxin system toxin CcdB Toxin
GRI03_RS00130 27657..27875 - 219 WP_095907066.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
GRI03_RS00135 28462..28696 + 235 Protein_26 hypothetical protein -
GRI03_RS00140 28719..29612 + 894 WP_187143874.1 hypothetical protein -
GRI03_RS24175 29678..29890 + 213 WP_187143868.1 hypothetical protein -
GRI03_RS00145 30137..31066 + 930 WP_095907164.1 Rpn family recombination-promoting nuclease/putative transposase -
GRI03_RS00150 31148..31729 - 582 WP_095907069.1 DUF2913 family protein -
GRI03_RS00155 31798..32061 - 264 Protein_31 NIPSNAP family protein -
GRI03_RS00160 32085..32318 - 234 WP_187143869.1 DUF2767 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..169226 169226


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11712.46 Da        Isoelectric Point: 6.2213

>T66846 WP_095907065.1 NZ_CP017473:c27655-27350 [Enterobacter cloacae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLTNAHLLSDKVSRELYPVVHIGDESWRLMTTDMASVPATVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T66846 NZ_CP017473:c27655-27350 [Enterobacter cloacae]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTACCGCCTGTTTGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGACGGCGTATGGTGATCCCGCTGACCAATGCCCATCTGCTGTCGGATAAGGTCTCCCGTGAGCTTTATCCGGTGG
TGCATATCGGGGATGAGAGCTGGCGTCTGATGACCACGGATATGGCCAGTGTCCCGGCCACAGTCATCGGGGAAGAGGTG
GCAGATCTCAGCCACAGGGAGAACGACATCAAAAACGCTATTAACCTGATGTTCTGGGGGATCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8275.21 Da        Isoelectric Point: 5.0651

>AT66846 WP_095907066.1 NZ_CP017473:c27875-27657 [Enterobacter cloacae]
MKQRITVTIDSDSYRLLKSADVNISGLVNTAMQKEARRLRAERWQAENQEGMAEVARFIDANGSFADENRDW

Download         Length: 219 bp

>AT66846 NZ_CP017473:c27875-27657 [Enterobacter cloacae]
ATGAAGCAGCGCATTACTGTCACCATCGACAGTGACAGCTACCGGTTACTCAAGTCTGCTGACGTGAATATCTCTGGCCT
GGTCAATACTGCCATGCAGAAGGAGGCGCGGCGTCTGCGTGCGGAACGCTGGCAGGCGGAAAATCAGGAAGGGATGGCCG
AGGTCGCCCGGTTTATTGACGCGAACGGCTCGTTTGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References