Detailed information of TA system TA066826

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2675032..2676466
• Replicon: chromosome
• Accession: NZ_CP017458
• Organism: Legionella pneumophila subsp. fraseri strain Dallas 1E

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: A0A2S6EXC5
• Locus tag: BIZ51_RS11930
• Protein ID: WP_032830191.1
• Coordinates: 2675246..2676466 (+)
• Length: 407 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: BIZ51_RS11925
• Protein ID: WP_027227407.1
• Coordinates: 2675032..2675259 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BIZ51_RS11900	2670827..2671693	-	867	WP_027227403.1	transposase	-
BIZ51_RS11905	2671755..2671943	-	189	WP_071974648.1	helix-turn-helix domain-containing protein	-
BIZ51_RS11910	2672132..2672920	+	789	WP_027227404.1	hypothetical protein	-
BIZ51_RS11915	2672985..2673395	-	411	Protein_2314	transposase family protein	-
BIZ51_RS11920	2673514..2674536	-	1023	WP_027227406.1	hypothetical protein	-
BIZ51_RS11925	2675032..2675259	+	228	WP_027227407.1	helix-turn-helix transcriptional regulator	Antitoxin
BIZ51_RS11930	2675246..2676466	+	1221	WP_032830191.1	type II toxin-antitoxin system HipA family toxin	Toxin
BIZ51_RS11935	2676501..2676857	-	357	WP_027227408.1	preprotein translocase	-
BIZ51_RS11940	2676897..2677802	-	906	WP_027227409.1	LysR family transcriptional regulator	-
BIZ51_RS11945	2677893..2678753	+	861	WP_027227410.1	DUF2608 domain-containing protein	-
BIZ51_RS11950	2679105..2679369	-	265	Protein_2321	hypothetical protein	-
BIZ51_RS11955	2679417..2679728	-	312	WP_027227411.1	cupin	-
BIZ51_RS11960	2679744..2679992	-	249	WP_031301595.1	hypothetical protein	-
BIZ51_RS11965	2680299..2680772	+	474	WP_027227413.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (147-380)

Antitoxin

• Transcription regulator, HipB-like (7-63)

Sequences

Toxin

Protein

• Length: 407 a.a.
• Molecular weight: 45374.54 Da
• Isoelectric Point: 8.3174

>T66826 WP_032830191.1 NZ_CP017458:2675246-2676466 [Legionella pneumophila subsp. fraseri]
MKNHKLDIYLNQTLAGQLRIDTHGEMSFVYDEGYLENNKNLPLSHSLPLQQAPYSTKQCRPFFSGLLPEAHMRTAIARQL
GISEKNDFALLAAIGGECAGAVMILPQDTSITQLKPDYKIIKKQDVLEILQTMHQRPMIVGEDGIRLSLAGAQDKLPVAI
IEGNLAIPMNGAPSTHILKPINRDFPSLIENESFCLNLAQKIGLNAVDASIHYADGTPYLLVKRYDRVETKQGIQRLHQE
DFCQAMGISPEMKYQREGGPQISECFGLIREISSLPVLDIKELLQGILFNVIIGNNDAHSKNFSLLYHGSKVRLAPFYDL
ISTVYYPNLASKMAMKVGSKYDFDGLFPRHFIQMAEEARLSGALVRKEVLNLISKIQKNITASPFTSIILQRADKLFQKF
QKASTG

Nucleotide

• Length: 1221 bp

>T66826 NZ_CP017458:2675246-2676466 [Legionella pneumophila subsp. fraseri]
ATGAAAAATCATAAACTTGATATCTATCTTAACCAAACACTAGCAGGCCAATTACGTATCGATACGCATGGCGAGATGTC
TTTTGTGTATGATGAAGGCTACCTGGAAAACAATAAAAATCTCCCGTTGTCACACTCCCTTCCCCTTCAACAAGCCCCTT
ACTCAACGAAGCAATGCCGTCCGTTTTTTAGCGGACTATTGCCTGAAGCCCATATGCGTACTGCTATTGCCCGTCAATTG
GGTATCAGTGAAAAAAATGATTTTGCCTTATTAGCAGCTATTGGTGGTGAGTGTGCCGGAGCTGTGATGATATTACCTCA
AGATACATCCATAACTCAGCTAAAGCCAGACTATAAAATTATCAAAAAACAGGATGTATTAGAAATCCTGCAAACCATGC
ATCAAAGACCCATGATAGTCGGTGAGGATGGAATTCGTTTATCCCTTGCTGGAGCTCAGGATAAACTTCCAGTTGCCATT
ATTGAAGGAAATCTCGCAATACCAATGAACGGTGCACCGAGTACTCATATTTTAAAACCAATTAATCGAGACTTTCCCTC
CTTAATTGAAAATGAATCTTTCTGCCTTAATCTGGCACAAAAAATTGGATTAAATGCTGTGGACGCATCAATCCATTATG
CTGATGGTACCCCTTATCTGTTGGTTAAACGTTATGATCGGGTTGAGACAAAGCAAGGAATCCAACGTCTTCATCAAGAA
GATTTTTGTCAGGCAATGGGCATTAGCCCTGAAATGAAATATCAGCGAGAAGGCGGCCCCCAAATTAGTGAGTGCTTTGG
TTTGATAAGAGAGATATCAAGCCTCCCTGTCCTTGACATAAAAGAATTATTACAAGGGATTCTGTTTAATGTCATTATTG
GCAACAATGATGCACATAGCAAAAACTTCTCTTTGCTCTATCATGGTTCAAAAGTTCGTTTAGCACCTTTCTACGATCTG
ATTTCAACCGTATATTATCCTAATCTTGCCAGTAAAATGGCGATGAAAGTTGGAAGTAAATATGATTTTGATGGCCTATT
TCCACGCCACTTTATACAAATGGCAGAGGAGGCTCGTTTATCGGGTGCCTTAGTGCGTAAAGAAGTCTTGAATCTAATTA
GCAAAATTCAAAAGAACATAACCGCCAGCCCCTTTACCAGCATCATTTTGCAACGTGCTGACAAATTATTTCAAAAGTTC
CAGAAGGCTTCAACAGGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8467.85 Da
• Isoelectric Point: 9.1200

>AT66826 WP_027227407.1 NZ_CP017458:2675032-2675259 [Legionella pneumophila subsp. fraseri]
MIQSPENIGRYIRKVRKELNVTQKDLALTAGTGLRFIIDLENGKPTCQIGKMLQVLQVLGIQLNLSHTDFENEKS

Nucleotide

• Length: 228 bp

>AT66826 NZ_CP017458:2675032-2675259 [Legionella pneumophila subsp. fraseri]
ATGATTCAATCTCCAGAAAATATAGGACGTTATATTCGCAAAGTTCGTAAAGAACTTAATGTTACCCAAAAAGATTTGGC
TTTAACTGCTGGAACTGGATTGCGTTTTATTATCGATTTGGAAAATGGAAAGCCCACTTGCCAAATTGGAAAAATGCTGC
AAGTACTACAAGTACTTGGGATTCAACTCAACTTATCTCATACTGATTTCGAAAATGAAAAATCATAA

Structures

Toxin

• AlphaFold DB: A0A2S6EXC5