Detailed information of TA system TA066824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2697807..2699241
• Replicon: chromosome
• Accession: NZ_CP017457
• Organism: Legionella pneumophila subsp. fraseri strain Detroit-1

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: A0A2S6EXC5
• Locus tag: BIZ52_RS12020
• Protein ID: WP_032830191.1
• Coordinates: 2698021..2699241 (+)
• Length: 407 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: BIZ52_RS12015
• Protein ID: WP_027227407.1
• Coordinates: 2697807..2698034 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BIZ52_RS11990	2693602..2694468	-	867	WP_027227403.1	transposase	-
BIZ52_RS11995	2694530..2694718	-	189	WP_071974648.1	helix-turn-helix domain-containing protein	-
BIZ52_RS12000	2694907..2695695	+	789	WP_027227404.1	hypothetical protein	-
BIZ52_RS12005	2695760..2696170	-	411	Protein_2332	transposase family protein	-
BIZ52_RS12010	2696289..2697311	-	1023	WP_027227406.1	hypothetical protein	-
BIZ52_RS12015	2697807..2698034	+	228	WP_027227407.1	helix-turn-helix transcriptional regulator	Antitoxin
BIZ52_RS12020	2698021..2699241	+	1221	WP_032830191.1	type II toxin-antitoxin system HipA family toxin	Toxin
BIZ52_RS12025	2699276..2699632	-	357	WP_027227408.1	preprotein translocase	-
BIZ52_RS12030	2699672..2700577	-	906	WP_027227409.1	LysR family transcriptional regulator	-
BIZ52_RS12035	2700668..2701528	+	861	WP_027227410.1	DUF2608 domain-containing protein	-
BIZ52_RS12040	2701880..2702144	-	265	Protein_2339	hypothetical protein	-
BIZ52_RS12045	2702192..2702503	-	312	WP_027227411.1	cupin	-
BIZ52_RS12050	2702519..2702767	-	249	WP_031301595.1	hypothetical protein	-
BIZ52_RS12055	2703074..2703547	+	474	WP_027227413.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (147-380)

Antitoxin

• Transcription regulator, HipB-like (7-63)

Sequences

Toxin

Protein

• Length: 407 a.a.
• Molecular weight: 45374.54 Da
• Isoelectric Point: 8.3174

>T66824 WP_032830191.1 NZ_CP017457:2698021-2699241 [Legionella pneumophila subsp. fraseri]
MKNHKLDIYLNQTLAGQLRIDTHGEMSFVYDEGYLENNKNLPLSHSLPLQQAPYSTKQCRPFFSGLLPEAHMRTAIARQL
GISEKNDFALLAAIGGECAGAVMILPQDTSITQLKPDYKIIKKQDVLEILQTMHQRPMIVGEDGIRLSLAGAQDKLPVAI
IEGNLAIPMNGAPSTHILKPINRDFPSLIENESFCLNLAQKIGLNAVDASIHYADGTPYLLVKRYDRVETKQGIQRLHQE
DFCQAMGISPEMKYQREGGPQISECFGLIREISSLPVLDIKELLQGILFNVIIGNNDAHSKNFSLLYHGSKVRLAPFYDL
ISTVYYPNLASKMAMKVGSKYDFDGLFPRHFIQMAEEARLSGALVRKEVLNLISKIQKNITASPFTSIILQRADKLFQKF
QKASTG

Nucleotide

• Length: 1221 bp

>T66824 NZ_CP017457:2698021-2699241 [Legionella pneumophila subsp. fraseri]
ATGAAAAATCATAAACTTGATATCTATCTTAACCAAACACTAGCAGGCCAATTACGTATCGATACGCATGGCGAGATGTC
TTTTGTGTATGATGAAGGCTACCTGGAAAACAATAAAAATCTCCCGTTGTCACACTCCCTTCCCCTTCAACAAGCCCCTT
ACTCAACGAAGCAATGCCGTCCGTTTTTTAGCGGACTATTGCCTGAAGCCCATATGCGTACTGCTATTGCCCGTCAATTG
GGTATCAGTGAAAAAAATGATTTTGCCTTATTAGCAGCTATTGGTGGTGAGTGTGCCGGAGCTGTGATGATATTACCTCA
AGATACATCCATAACTCAGCTAAAGCCAGACTATAAAATTATCAAAAAACAGGATGTATTAGAAATCCTGCAAACCATGC
ATCAAAGACCCATGATAGTCGGTGAGGATGGAATTCGTTTATCCCTTGCTGGAGCTCAGGATAAACTTCCAGTTGCCATT
ATTGAAGGAAATCTCGCAATACCAATGAACGGTGCACCGAGTACTCATATTTTAAAACCAATTAATCGAGACTTTCCCTC
CTTAATTGAAAATGAATCTTTCTGCCTTAATCTGGCACAAAAAATTGGATTAAATGCTGTGGACGCATCAATCCATTATG
CTGATGGTACCCCTTATCTGTTGGTTAAACGTTATGATCGGGTTGAGACAAAGCAAGGAATCCAACGTCTTCATCAAGAA
GATTTTTGTCAGGCAATGGGCATTAGCCCTGAAATGAAATATCAGCGAGAAGGCGGCCCCCAAATTAGTGAGTGCTTTGG
TTTGATAAGAGAGATATCAAGCCTCCCTGTCCTTGACATAAAAGAATTATTACAAGGGATTCTGTTTAATGTCATTATTG
GCAACAATGATGCACATAGCAAAAACTTCTCTTTGCTCTATCATGGTTCAAAAGTTCGTTTAGCACCTTTCTACGATCTG
ATTTCAACCGTATATTATCCTAATCTTGCCAGTAAAATGGCGATGAAAGTTGGAAGTAAATATGATTTTGATGGCCTATT
TCCACGCCACTTTATACAAATGGCAGAGGAGGCTCGTTTATCGGGTGCCTTAGTGCGTAAAGAAGTCTTGAATCTAATTA
GCAAAATTCAAAAGAACATAACCGCCAGCCCCTTTACCAGCATCATTTTGCAACGTGCTGACAAATTATTTCAAAAGTTC
CAGAAGGCTTCAACAGGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8467.85 Da
• Isoelectric Point: 9.1200

>AT66824 WP_027227407.1 NZ_CP017457:2697807-2698034 [Legionella pneumophila subsp. fraseri]
MIQSPENIGRYIRKVRKELNVTQKDLALTAGTGLRFIIDLENGKPTCQIGKMLQVLQVLGIQLNLSHTDFENEKS

Nucleotide

• Length: 228 bp

>AT66824 NZ_CP017457:2697807-2698034 [Legionella pneumophila subsp. fraseri]
ATGATTCAATCTCCAGAAAATATAGGACGTTATATTCGCAAAGTTCGTAAAGAACTTAATGTTACCCAAAAAGATTTGGC
TTTAACTGCTGGAACTGGATTGCGTTTTATTATCGATTTGGAAAATGGAAAGCCCACTTGCCAAATTGGAAAAATGCTGC
AAGTACTACAAGTACTTGGGATTCAACTCAACTTATCTCATACTGATTTCGAAAATGAAAAATCATAA

Structures

Toxin

• AlphaFold DB: A0A2S6EXC5