Detailed information of TA system TA066821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2157213..2157997
• Replicon: chromosome
• Accession: NZ_CP017454
• Organism: Dickeya solani strain PPO 9019

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: BJJ98_RS09460
• Protein ID: WP_022632047.1
• Coordinates: 2157213..2157707 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A2K8W1V4
• Locus tag: BJJ98_RS09465
• Protein ID: WP_022632046.1
• Coordinates: 2157704..2157997 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJJ98_RS09440	2152879..2154819	-	1941	WP_022632050.1	hypothetical protein	-
BJJ98_RS09445	2154807..2155373	-	567	WP_024103901.1	hypothetical protein	-
BJJ98_RS09450	2155377..2156648	-	1272	WP_022632048.1	hypothetical protein	-
BJJ98_RS09455	2156764..2157161	-	398	Protein_1839	DUF3577 domain-containing protein	-
BJJ98_RS09460	2157213..2157707	-	495	WP_022632047.1	GNAT family N-acetyltransferase	Toxin
BJJ98_RS09465	2157704..2157997	-	294	WP_022632046.1	DUF1778 domain-containing protein	Antitoxin
BJJ98_RS09470	2158402..2159653	+	1252	Protein_1842	tyrosine-type recombinase/integrase	-
BJJ98_RS09475	2160645..2161352	-	708	WP_022632044.1	SDR family oxidoreductase	-
BJJ98_RS09480	2161380..2161676	-	297	WP_022632043.1	hypothetical protein	-
BJJ98_RS09485	2161719..2162171	-	453	WP_022632042.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2147921..2158240	10319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-95)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17639.41 Da
• Isoelectric Point: 6.8524

>T66821 WP_022632047.1 NZ_CP017454:c2157707-2157213 [Dickeya solani]
MISAPEPLHAEHVLSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDDSRVLAYYSLASSAVATTAAPGRFRRNMPDPI
PVVVLGRLAVDTSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEARDFYLRVGFEPSPIDPMILMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T66821 NZ_CP017454:c2157707-2157213 [Dickeya solani]
ATGATCTCTGCTCCTGAGCCACTCCACGCCGAACATGTACTATCTTCATTCTGTTGCGGTGTGGAGTCTATGGACAACTG
GCTGAAACAACGGGCGATGAAAAATCAGGTCACTGGCGCATCCCGAACTTTCGTCAGTTGCGATGATTCAAGAGTGTTGG
CGTATTACTCATTGGCTTCCAGCGCCGTGGCAACAACTGCCGCCCCCGGACGCTTTCGTCGCAATATGCCCGATCCGATC
CCGGTGGTAGTGCTGGGACGTCTGGCGGTTGATACGTCATTGCATGGTCAGGGCGTTGGCCGGGCGCTGGTGCGTGATGC
CGGGCTACGGGTGATTCAGGTAGCGGATACTATCGGCATTCGCGGGATGTTGGTTCATGCGCTGTCTGATGAAGCGCGTG
ATTTTTACCTACGAGTGGGGTTTGAACCTTCACCGATTGACCCGATGATTTTGATGGTAACGTTGGGGGATTTGGTGGGG
AGTTTATCAATTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10942.53 Da
• Isoelectric Point: 9.8078

>AT66821 WP_022632046.1 NZ_CP017454:c2157997-2157704 [Dickeya solani]
MPVTNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPEPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT66821 NZ_CP017454:c2157997-2157704 [Dickeya solani]
ATGCCAGTGACGAACAGCGTTTCTGTTAAACGCGAAACATTGAATTTACGCATCAAGCCCGCCGAACGCGATCTGATCGA
TCGTGCAGCGAAGGCCAGAGGAAAAAATCGTACTGATTTTGTTCTTGAGGCTGCTCGCGCCGCCGCAGAGGAAGCGTTGA
TCGATCAGCGTATCATCATGGCAGATCCAGATGCCTATCAGGAATTCCTTGCTCGCTTGGATCAGGCTCCTGAGCCAAAT
GCCGCGCTGCGTAAAACCATGCAAACGCCTGCACCGTGGGAACAGAAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2K8W1V4