Detailed information of TA system TA066815

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 867746..868299
• Replicon: chromosome
• Accession: NZ_CP017454
• Organism: Dickeya solani strain PPO 9019

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: BJJ98_RS03815
• Protein ID: WP_022633101.1
• Coordinates: 867746..868060 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A2K8W593
• Locus tag: BJJ98_RS03820
• Protein ID: WP_022633100.1
• Coordinates: 868063..868299 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJJ98_RS03790	864473..865219	+	747	WP_022633104.1	hypothetical protein	-
BJJ98_RS03800	865602..866009	+	408	WP_071598628.1	helix-turn-helix transcriptional regulator	-
BJJ98_RS03805	866009..867316	+	1308	WP_022633103.1	type II toxin-antitoxin system HipA family toxin	-
BJJ98_RS03810	867492..867749	+	258	WP_022633102.1	hypothetical protein	-
BJJ98_RS03815	867746..868060	-	315	WP_022633101.1	CcdB family protein	Toxin
BJJ98_RS03820	868063..868299	-	237	WP_022633100.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
BJJ98_RS03825	868620..869564	-	945	WP_022633099.1	DUF1738 domain-containing protein	-
BJJ98_RS21965	869955..870248	+	294	WP_146053334.1	hypothetical protein	-
BJJ98_RS03830	870312..870713	+	402	WP_023637748.1	hypothetical protein	-
BJJ98_RS03835	871074..872723	-	1650	WP_022633097.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	hcp	828904..877282	48378

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11459.38 Da
• Isoelectric Point: 7.9859

>T66815 WP_022633101.1 NZ_CP017454:c868060-867746 [Dickeya solani]
MQFTVYGNTGKSAVYPLLLDVTSDIIGQLNRRIVIPLLPVEKYPAGRRPDRLVPVVRLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRTQVKAAIDFLIDGI

Nucleotide

• Length: 315 bp

>T66815 NZ_CP017454:c868060-867746 [Dickeya solani]
ATGCAATTCACCGTATACGGTAATACCGGGAAAAGCGCTGTTTACCCGCTGTTGCTCGATGTCACAAGCGATATTATTGG
GCAGTTGAATCGCCGGATAGTGATCCCGTTGCTCCCTGTTGAAAAGTATCCGGCAGGCCGCCGACCAGATCGTCTTGTCC
CCGTCGTCAGGCTGACGGACGGCAAAGAGTACGCCGTAATGACGCACGAGCTGGCAAGTATCCCTGTGCAGGCACTGGGG
GCGGTGTTTTGTGATGCGTCTCAGTATCGTACTCAGGTAAAGGCCGCAATAGATTTCCTCATCGACGGCATTTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9020.06 Da
• Isoelectric Point: 6.2206

>AT66815 WP_022633100.1 NZ_CP017454:c868299-868063 [Dickeya solani]
MTAKQRNTQSVTMTVERALLVRAREAGINLSAILTTALDAELRHHEAKKWQEENREAIDALNRFHDENGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT66815 NZ_CP017454:c868299-868063 [Dickeya solani]
ATGACCGCGAAACAACGCAACACGCAAAGCGTGACCATGACGGTGGAGCGTGCCTTACTGGTCCGGGCGCGTGAAGCGGG
CATTAACCTGAGTGCCATCCTGACAACCGCCCTGGATGCAGAGCTTCGCCACCATGAAGCAAAAAAATGGCAGGAAGAGA
ACAGGGAAGCCATCGACGCATTAAACCGTTTTCATGATGAAAATGGCTGTTTCAGTGATGAATACAGGACGTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2K8W593