Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 26592..27117 Replicon plasmid unnamed2
Accession NZ_CP017452
Organism Klebsiella sp. LTGPAF-6F

Toxin (Protein)


Gene name ccdB Uniprot ID A0A1D8K3Q5
Locus tag BJF97_RS31655 Protein ID WP_006788214.1
Coordinates 26812..27117 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag BJF97_RS31650 Protein ID WP_006788213.1
Coordinates 26592..26810 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJF97_RS31635 24429..25133 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
BJF97_RS31640 25321..25737 - 417 WP_021312768.1 type II toxin-antitoxin system VapC family toxin -
BJF97_RS31645 25734..25964 - 231 WP_021312767.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
BJF97_RS31650 26592..26810 + 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
BJF97_RS31655 26812..27117 + 306 WP_006788214.1 type II toxin-antitoxin system toxin CcdB Toxin
BJF97_RS34620 27803..28288 + 486 Protein_27 hypothetical protein -
BJF97_RS31665 28524..29318 + 795 WP_016240356.1 site-specific integrase -
BJF97_RS33845 29734..29913 - 180 WP_006788217.1 hypothetical protein -
BJF97_RS31670 30033..30659 - 627 WP_016946792.1 ParA family plasmid-partitioning AAA ATPase -
BJF97_RS31675 31236..32111 - 876 WP_004098982.1 RepB family plasmid replication initiator protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..89552 89552


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11687.46 Da        Isoelectric Point: 7.2015

>T66785 WP_006788214.1 NZ_CP017452:26812-27117 [Klebsiella sp. LTGPAF-6F]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRLMTTDMASVPASVTGEEV
ADLRHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T66785 NZ_CP017452:26812-27117 [Klebsiella sp. LTGPAF-6F]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGGCGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAGCTCTATCCGGTGG
TGCACATCGGGGACGAGAGCTGGCGCCTGATGACCACGGACATGGCCAGCGTCCCGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCCGCCACCGGGAAAATGACATCAAAAACGCAATTAACCTGATGTTCTGGGGGATCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT66785 WP_006788213.1 NZ_CP017452:26592-26810 [Klebsiella sp. LTGPAF-6F]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT66785 NZ_CP017452:26592-26810 [Klebsiella sp. LTGPAF-6F]
ATGAAGCAGCGCATCACCGTCACCATCGACAGCGACAGCTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAAGCCCGACGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCTG
AGGTTGCCCGGTTTATTGAAATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8K3Q5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References