Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 25321..25964 Replicon plasmid unnamed2
Accession NZ_CP017452
Organism Klebsiella sp. LTGPAF-6F

Toxin (Protein)


Gene name vagD Uniprot ID A0A5P6KDG5
Locus tag BJF97_RS31640 Protein ID WP_021312768.1
Coordinates 25321..25737 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A5P6KGW8
Locus tag BJF97_RS31645 Protein ID WP_021312767.1
Coordinates 25734..25964 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJF97_RS31625 20414..21382 + 969 WP_077255425.1 IS5 family transposase -
BJF97_RS31630 21590..24364 - 2775 Protein_21 Tn3 family transposase -
BJF97_RS31635 24429..25133 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
BJF97_RS31640 25321..25737 - 417 WP_021312768.1 type II toxin-antitoxin system VapC family toxin Toxin
BJF97_RS31645 25734..25964 - 231 WP_021312767.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BJF97_RS31650 26592..26810 + 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA -
BJF97_RS31655 26812..27117 + 306 WP_006788214.1 type II toxin-antitoxin system toxin CcdB -
BJF97_RS34620 27803..28288 + 486 Protein_27 hypothetical protein -
BJF97_RS31665 28524..29318 + 795 WP_016240356.1 site-specific integrase -
BJF97_RS33845 29734..29913 - 180 WP_006788217.1 hypothetical protein -
BJF97_RS31670 30033..30659 - 627 WP_016946792.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..89552 89552


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15175.60 Da        Isoelectric Point: 7.2452

>T66784 WP_021312768.1 NZ_CP017452:c25737-25321 [Klebsiella sp. LTGPAF-6F]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPDLVLEDWVR

Download         Length: 417 bp

>T66784 NZ_CP017452:c25737-25321 [Klebsiella sp. LTGPAF-6F]
GTGAACAAGATCTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGTGGCCACCGTATCGTGGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGTGCCACCGGCC
CGAAGGCCTCGCCGCGCCACGTCCAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGACCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGCCTCGCCGGAACGCCGATAGGCCCGAACGACACGGCGATCGC
CGGGCATGCCATTGCGGCCGGCGCCGTACTGGTGACAAATAATGTGCGGGAGTTTGAGCGGGTGCCGGATCTGGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8722.81 Da        Isoelectric Point: 4.4692

>AT66784 WP_021312767.1 NZ_CP017452:c25964-25734 [Klebsiella sp. LTGPAF-6F]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT66784 NZ_CP017452:c25964-25734 [Klebsiella sp. LTGPAF-6F]
ATGAGAACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGGTCGTTCGCGCAGCTCG
AAAAAGCCGATCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5P6KDG5

Antitoxin

Source ID Structure
AlphaFold DB A0A5P6KGW8

References