Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 279412..279934 Replicon plasmid unnamed1
Accession NZ_CP017451
Organism Klebsiella sp. LTGPAF-6F

Toxin (Protein)


Gene name stbE Uniprot ID A0A2J4R0S6
Locus tag BJF97_RS30250 Protein ID WP_004181778.1
Coordinates 279412..279696 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag BJF97_RS30255 Protein ID WP_004181777.1
Coordinates 279686..279934 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJF97_RS33500 274613..275708 + 1096 Protein_271 conjugal transfer protein TraG N-terminal domain-containing protein -
BJF97_RS30240 275807..276994 - 1188 WP_040209642.1 transposase -
BJF97_RS33505 277030..277458 + 429 WP_077257669.1 IS200/IS605 family transposase -
BJF97_RS33510 277537..277761 + 225 Protein_274 transposase -
BJF97_RS30245 278032..279258 - 1227 WP_040209639.1 transposase -
BJF97_RS34505 279294..279395 + 102 Protein_276 IS200/IS605 family transposase -
BJF97_RS30250 279412..279696 - 285 WP_004181778.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BJF97_RS30255 279686..279934 - 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BJF97_RS30260 280225..282024 + 1800 WP_004181776.1 ATP-dependent helicase -
BJF97_RS30265 282358..283344 + 987 WP_025368599.1 hypothetical protein -
BJF97_RS30270 283489..283842 + 354 WP_004181774.1 hypothetical protein -
BJF97_RS30275 283904..284725 + 822 WP_004181772.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3'')-Ib / aph(6)-Id / formA - 1..526446 526446


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10970.69 Da        Isoelectric Point: 10.6516

>T66783 WP_004181778.1 NZ_CP017451:c279696-279412 [Klebsiella sp. LTGPAF-6F]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Download         Length: 285 bp

>T66783 NZ_CP017451:c279696-279412 [Klebsiella sp. LTGPAF-6F]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT66783 WP_004181777.1 NZ_CP017451:c279934-279686 [Klebsiella sp. LTGPAF-6F]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT66783 NZ_CP017451:c279934-279686 [Klebsiella sp. LTGPAF-6F]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4R0S6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References