Detailed information of TA system TA066782

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 134972..135882
• Replicon: plasmid unnamed1
• Accession: NZ_CP017451
• Organism: Klebsiella sp. LTGPAF-6F

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A1D8K2L2
• Locus tag: BJF97_RS29550
• Protein ID: WP_070083042.1
• Coordinates: 134972..135442 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: BJF97_RS29555
• Protein ID: WP_004181895.1
• Coordinates: 135439..135882 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJF97_RS29530	130226..131566	-	1341	WP_004196353.1	ISNCY family transposase	-
BJF97_RS29535	131641..133173	-	1533	WP_009310015.1	IS3-like element ISKpn38 family transposase	-
BJF97_RS29540	133215..134039	+	825	Protein_133	transposase	-
BJF97_RS29545	134601..134861	+	261	WP_070083041.1	hypothetical protein	-
BJF97_RS29550	134972..135442	-	471	WP_070083042.1	RES family NAD+ phosphorylase	Toxin
BJF97_RS29555	135439..135882	-	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
BJF97_RS29560	135983..137254	-	1272	WP_004197886.1	translesion error-prone DNA polymerase V subunit UmuC	-
BJF97_RS29565	137254..137676	-	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
BJF97_RS29570	137973..138670	+	698	Protein_139	IS1 family transposase	-
BJF97_RS33405	138664..139159	-	496	Protein_140	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3'')-Ib / aph(6)-Id / formA	-	1..526446	526446
	-	inside	IScluster/Tn	-	-	131641..153154	21513

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17587.85 Da
• Isoelectric Point: 4.5244

>T66782 WP_070083042.1 NZ_CP017451:c135442-134972 [Klebsiella sp. LTGPAF-6F]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMESFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T66782 NZ_CP017451:c135442-134972 [Klebsiella sp. LTGPAF-6F]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGAATCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCTCTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATTGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT66782 WP_004181895.1 NZ_CP017451:c135882-135439 [Klebsiella sp. LTGPAF-6F]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT66782 NZ_CP017451:c135882-135439 [Klebsiella sp. LTGPAF-6F]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A1D8K2L2

Antitoxin

• AlphaFold DB: A0A2J4RLY9