Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 6074482..6075029 Replicon chromosome
Accession NZ_CP017450
Organism Klebsiella sp. LTGPAF-6F

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag BJF97_RS28460 Protein ID WP_014227299.1
Coordinates 6074721..6075029 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag BJF97_RS28455 Protein ID WP_014227298.1
Coordinates 6074482..6074718 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJF97_RS28440 6070689..6071855 + 1167 WP_014227296.1 multidrug effflux MFS transporter -
BJF97_RS28445 6072016..6072918 + 903 WP_009654080.1 N-acetylmuramic acid 6-phosphate etherase -
BJF97_RS28450 6072943..6074376 + 1434 WP_014227297.1 PTS N-acetylmuramic acid transporter subunit IIBC -
BJF97_RS28455 6074482..6074718 + 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
BJF97_RS28460 6074721..6075029 + 309 WP_014227299.1 CcdB family protein Toxin
BJF97_RS28465 6075180..6076082 + 903 WP_014227300.1 EamA family transporter -
BJF97_RS28470 6076086..6076343 - 258 Protein_5632 hypothetical protein -
BJF97_RS28475 6076343..6077308 - 966 WP_038423881.1 Rpn family recombination-promoting nuclease/putative transposase -
BJF97_RS28480 6077518..6077811 + 294 WP_014227302.1 hypothetical protein -
BJF97_RS28485 6077835..6078776 - 942 WP_014227303.1 LacI family transcriptional regulator -
BJF97_RS28490 6079071..6079382 + 312 WP_014227304.1 PTS sugar transporter subunit IIB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T66779 WP_014227299.1 NZ_CP017450:6074721-6075029 [Klebsiella sp. LTGPAF-6F]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T66779 NZ_CP017450:6074721-6075029 [Klebsiella sp. LTGPAF-6F]
ATGCAGTATTACGTTTATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CAAGCGCAATACTCGGGTCGTGATTCCACTGTTTCCACTGAAAAACTATAAAGGTCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACATCGTGTTCTGGGTGAAGAA
GTTTGTAATCTGAATCACCAGCGCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT66779 WP_014227298.1 NZ_CP017450:6074482-6074718 [Klebsiella sp. LTGPAF-6F]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT66779 NZ_CP017450:6074482-6074718 [Klebsiella sp. LTGPAF-6F]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCAGGCCTGGTCAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCAGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References