Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 4011992..4012582 Replicon chromosome
Accession NZ_CP017450
Organism Klebsiella sp. LTGPAF-6F

Toxin (Protein)


Gene name pemK Uniprot ID A0A1D8JW90
Locus tag BJF97_RS18750 Protein ID WP_032693748.1
Coordinates 4011992..4012324 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag BJF97_RS18755 Protein ID WP_004852307.1
Coordinates 4012325..4012582 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJF97_RS18725 4007404..4007661 + 258 WP_014229908.1 histidine kinase -
BJF97_RS18730 4007794..4009248 + 1455 WP_014229909.1 AMP nucleosidase -
BJF97_RS18740 4010212..4011669 - 1458 WP_014229916.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
BJF97_RS18750 4011992..4012324 - 333 WP_032693748.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BJF97_RS18755 4012325..4012582 - 258 WP_004852307.1 antitoxin Antitoxin
BJF97_RS18760 4012843..4013036 - 194 Protein_3699 helix-turn-helix domain-containing protein -
BJF97_RS18765 4013033..4013506 - 474 WP_050595132.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11752.67 Da        Isoelectric Point: 10.4751

>T66776 WP_032693748.1 NZ_CP017450:c4012324-4011992 [Klebsiella sp. LTGPAF-6F]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLARLPVVVPITSGGKFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMVARNGKRLESIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T66776 NZ_CP017450:c4012324-4011992 [Klebsiella sp. LTGPAF-6F]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTGGATCCGATTGCAGGCCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGGCCCGGTTACCCGTTGTTGTTCCCATCACCAGCGGTGGAAAATTTGCTCGCA
CGGCAGGTTTCACCGTTTCACTGGAAGGGGCAGGGACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGTTGCCCGGAACGGCAAGCGGCTGGAAAGCATACCAGACGCTGTGGTAAATGAAGTGCTTGCCAGGCTGGATGC
AATTTTAAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT66776 WP_004852307.1 NZ_CP017450:c4012582-4012325 [Klebsiella sp. LTGPAF-6F]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT66776 NZ_CP017450:c4012582-4012325 [Klebsiella sp. LTGPAF-6F]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGCTCCATCATGTTGGCGGTTCCTCCCGCCGTGCTCAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTCGGCATGACCATTAATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCTCATTATTCTC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATAGGGAATGGATTGATGCTCCTGCGGTG
GGCAAGGAGATCCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8JW90

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References