Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1552264..1552883 Replicon chromosome
Accession NZ_CP017450
Organism Klebsiella sp. LTGPAF-6F

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag BJF97_RS07275 Protein ID WP_004099646.1
Coordinates 1552264..1552482 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag BJF97_RS07280 Protein ID WP_004129911.1
Coordinates 1552509..1552883 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJF97_RS07245 1548309..1548572 + 264 WP_004848323.1 type B 50S ribosomal protein L31 -
BJF97_RS07250 1548572..1548712 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
BJF97_RS07255 1548709..1549407 - 699 WP_014228203.1 GNAT family N-acetyltransferase -
BJF97_RS07260 1549509..1550963 + 1455 WP_032694158.1 PLP-dependent aminotransferase family protein -
BJF97_RS07265 1550938..1551408 - 471 WP_014228205.1 YlaC family protein -
BJF97_RS07270 1551535..1552101 - 567 WP_032694159.1 maltose O-acetyltransferase -
BJF97_RS07275 1552264..1552482 - 219 WP_004099646.1 hemolysin expression modulator Hha Toxin
BJF97_RS07280 1552509..1552883 - 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
BJF97_RS07285 1553364..1556510 - 3147 WP_070082856.1 multidrug efflux RND transporter permease subunit AcrB -
BJF97_RS07290 1556533..1557726 - 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T66767 WP_004099646.1 NZ_CP017450:c1552482-1552264 [Klebsiella sp. LTGPAF-6F]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T66767 NZ_CP017450:c1552482-1552264 [Klebsiella sp. LTGPAF-6F]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCTGCGGCCGATCATCGTCTTGCAGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT66767 WP_004129911.1 NZ_CP017450:c1552883-1552509 [Klebsiella sp. LTGPAF-6F]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT66767 NZ_CP017450:c1552883-1552509 [Klebsiella sp. LTGPAF-6F]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGATCCAACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTTGCACTTAATTATAAAATTAAGTACGCAGAGGATAATAAGCTGATTACCCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References