Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 860963..861539 Replicon chromosome
Accession NZ_CP017450
Organism Klebsiella sp. LTGPAF-6F

Toxin (Protein)


Gene name shpA Uniprot ID A0A1D8JNH2
Locus tag BJF97_RS04095 Protein ID WP_042933975.1
Coordinates 860963..861250 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1D8JNF7
Locus tag BJF97_RS04100 Protein ID WP_025108476.1
Coordinates 861237..861539 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJF97_RS04065 856304..856822 - 519 WP_004098240.1 hypothetical protein -
BJF97_RS04070 856819..857619 - 801 WP_032693034.1 winged helix-turn-helix domain-containing protein -
BJF97_RS04075 857765..858349 - 585 WP_004098242.1 TetR/AcrR family transcriptional regulator -
BJF97_RS04080 858497..859279 - 783 WP_032693033.1 NAD(P)H-dependent oxidoreductase -
BJF97_RS04085 859367..860275 + 909 WP_032693031.1 LysR family transcriptional regulator -
BJF97_RS04090 860269..860712 + 444 WP_032693030.1 FosA family fosfomycin resistance glutathione transferase -
BJF97_RS04095 860963..861250 + 288 WP_042933975.1 BrnT family toxin Toxin
BJF97_RS04100 861237..861539 + 303 WP_025108476.1 BrnA antitoxin family protein Antitoxin
BJF97_RS04105 861660..863003 - 1344 WP_070082846.1 APC family permease -
BJF97_RS04110 863000..863911 - 912 WP_032693024.1 acetamidase/formamidase family protein -
BJF97_RS04115 864363..864698 - 336 WP_014227760.1 endoribonuclease SymE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11239.69 Da        Isoelectric Point: 8.5899

>T66765 WP_042933975.1 NZ_CP017450:860963-861250 [Klebsiella sp. LTGPAF-6F]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGVIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Download         Length: 288 bp

>T66765 NZ_CP017450:860963-861250 [Klebsiella sp. LTGPAF-6F]
ATGCCAATGGAGTTTGAATGGGATGCGAACAAGGCAAGAAGTAATCTAAGAAAACATGGAATCCGGTTTGAAGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTATCGGTC
TCGTTCATGGGGTCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11563.17 Da        Isoelectric Point: 9.7962

>AT66765 WP_025108476.1 NZ_CP017450:861237-861539 [Klebsiella sp. LTGPAF-6F]
MNMVKHKRDNPDTLTAKRADELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp

>AT66765 NZ_CP017450:861237-861539 [Klebsiella sp. LTGPAF-6F]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGCCGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8JNH2

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8JNF7

References