Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 443196..443953 Replicon chromosome
Accession NZ_CP017450
Organism Klebsiella sp. LTGPAF-6F

Toxin (Protein)


Gene name tacT Uniprot ID A0A1D8JMF4
Locus tag BJF97_RS02090 Protein ID WP_044159004.1
Coordinates 443471..443953 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A1D8JMH3
Locus tag BJF97_RS02085 Protein ID WP_007372584.1
Coordinates 443196..443480 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJF97_RS02065 438588..439310 + 723 WP_013095151.1 TIGR03761 family integrating conjugative element protein -
BJF97_RS02070 439328..441340 + 2013 WP_044159002.1 DNA topoisomerase III -
BJF97_RS02075 441973..442464 + 492 WP_007372582.1 DUF3577 domain-containing protein -
BJF97_RS02080 442540..443040 + 501 WP_007372583.1 single-stranded DNA-binding protein -
BJF97_RS02085 443196..443480 + 285 WP_007372584.1 DUF1778 domain-containing protein Antitoxin
BJF97_RS02090 443471..443953 + 483 WP_044159004.1 GNAT family N-acetyltransferase Toxin
BJF97_RS02095 444186..445471 + 1286 Protein_388 TcpQ domain-containing protein -
BJF97_RS02100 445473..445916 + 444 WP_007372587.1 type IV pilus biogenesis protein PilM -
BJF97_RS02105 445936..447609 + 1674 WP_013095157.1 PilN family type IVB pilus formation outer membrane protein -
BJF97_RS02110 447612..448892 + 1281 WP_176682058.1 type 4b pilus protein PilO2 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - pilL 437436..546958 109522


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(39-146)

Antitoxin

Vibrio phage ICP1, Orf50

(1-93)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17693.32 Da        Isoelectric Point: 6.9570

>T66764 WP_044159004.1 NZ_CP017450:443471-443953 [Klebsiella sp. LTGPAF-6F]
MEINVTAPALLTEEHVLHSFDCGNAVLDDWLRRRALKNQHLNASRTFVICPEGTACVVGYYSLATGSVSHVDLSRSLRQN
MPDPVPVVLLGRLAVDVSTQGNNFGKWLLNDAVMRVSNLADQVGIKAIMVHAIDERAKAFYEYFGFVQSPVAANTLFYKI

Download         Length: 483 bp

>T66764 NZ_CP017450:443471-443953 [Klebsiella sp. LTGPAF-6F]
ATGGAAATAAATGTCACTGCGCCTGCTTTACTGACAGAGGAGCATGTTCTTCATTCGTTCGATTGCGGGAATGCGGTACT
TGATGACTGGCTTCGCCGGCGTGCGTTGAAGAATCAGCATCTTAATGCTTCCCGGACATTCGTCATCTGTCCTGAAGGTA
CTGCGTGTGTAGTGGGTTATTATTCCCTTGCGACGGGCTCAGTTAGTCATGTCGACTTGAGTCGTAGTCTGCGACAGAAT
ATGCCCGATCCCGTACCGGTTGTTTTGCTGGGCCGCCTTGCAGTCGATGTATCCACTCAGGGCAATAACTTCGGGAAATG
GTTGCTGAATGATGCTGTGATGCGCGTATCGAATCTGGCGGATCAGGTCGGCATCAAGGCTATCATGGTGCATGCAATTG
ATGAGCGGGCTAAAGCGTTCTATGAATACTTTGGTTTTGTGCAGTCACCGGTTGCGGCTAACACGCTTTTCTACAAAATA
TAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 11078.63 Da        Isoelectric Point: 8.5684

>AT66764 WP_007372584.1 NZ_CP017450:443196-443480 [Klebsiella sp. LTGPAF-6F]
MQTPVRKSVRDKQINIRATDEERAVIDYAASLVNKNRTDFIIERAVHEAQNIILDQRVFVLDDARYQAFIRQLEAPVQNV
EGRQRLMDVKPEWK

Download         Length: 285 bp

>AT66764 NZ_CP017450:443196-443480 [Klebsiella sp. LTGPAF-6F]
ATGCAAACTCCAGTCCGTAAATCCGTCCGTGACAAGCAAATCAATATCCGCGCCACGGATGAAGAACGTGCGGTGATTGA
TTACGCTGCGAGTCTGGTTAATAAAAACCGTACTGATTTCATCATTGAAAGGGCTGTGCATGAGGCCCAGAACATCATTC
TGGACCAGCGTGTGTTCGTTCTTGATGATGCCCGTTACCAGGCGTTCATCAGGCAGCTTGAAGCCCCTGTGCAGAATGTT
GAAGGTCGTCAGCGACTGATGGATGTAAAACCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8JMF4

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8JMH3

References