Detailed information of TA system TA066752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3495458..3495936
• Replicon: chromosome
• Accession: NZ_CP017446
• Organism: Escherichia coli O157:H7 strain 9234

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: A4C47_RS18455
• Protein ID: WP_001303876.1
• Coordinates: 3495649..3495936 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: A4C47_RS18450
• Protein ID: WP_000536233.1
• Coordinates: 3495458..3495649 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4C47_RS18415	3490862..3491632	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
A4C47_RS18420	3491654..3492400	-	747	WP_000788751.1	ATP-binding protein	-
A4C47_RS18425	3492407..3493498	-	1092	WP_001205823.1	hypothetical protein	-
A4C47_RS29765	3493577..3494032	+	456	WP_000273724.1	hypothetical protein	-
A4C47_RS18435	3494239..3494664	-	426	WP_000693855.1	Rha family transcriptional regulator	-
A4C47_RS18440	3494648..3494920	-	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
A4C47_RS18445	3495029..3495430	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
A4C47_RS18450	3495458..3495649	+	192	WP_000536233.1	hypothetical protein	Antitoxin
A4C47_RS18455	3495649..3495936	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A4C47_RS18460	3496214..3496369	+	156	WP_000379575.1	DUF1391 family protein	-
A4C47_RS18465	3496511..3496834	+	324	WP_001458970.1	hypothetical protein	-
A4C47_RS18470	3497087..3497272	-	186	WP_001133046.1	hypothetical protein	-
A4C47_RS18480	3497846..3498034	+	189	WP_000413705.1	cell division inhibition protein DicB	-
A4C47_RS18485	3498031..3498222	+	192	WP_001098307.1	DUF1482 family protein	-
A4C47_RS18490	3498316..3500787	+	2472	WP_000034474.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3487995..3509449	21454

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T66752 WP_001303876.1 NZ_CP017446:3495649-3495936 [Escherichia coli O157:H7]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T66752 NZ_CP017446:3495649-3495936 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT66752 WP_000536233.1 NZ_CP017446:3495458-3495649 [Escherichia coli O157:H7]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT66752 NZ_CP017446:3495458-3495649 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA