Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 48858..49383 Replicon plasmid pO157
Accession NZ_CP017445
Organism Escherichia coli O157:H7 strain 8368

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag A4C39_RS28050 Protein ID WP_001159868.1
Coordinates 49078..49383 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag A4C39_RS28045 Protein ID WP_000813634.1
Coordinates 48858..49076 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4C39_RS30635 44141..44371 + 231 WP_010891288.1 hypothetical protein -
A4C39_RS28010 44492..45232 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
A4C39_RS28015 45517..46494 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
A4C39_RS28025 46902..47102 - 201 WP_000708307.1 hypothetical protein -
A4C39_RS28030 47099..47719 - 621 WP_001248529.1 hypothetical protein -
A4C39_RS28035 47716..48399 - 684 WP_010891291.1 DNA-binding protein -
A4C39_RS28045 48858..49076 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
A4C39_RS28050 49078..49383 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
A4C39_RS28055 49384..50190 + 807 WP_000016989.1 site-specific integrase -
A4C39_RS30645 52088..52426 + 339 WP_071525396.1 RepB family plasmid replication initiator protein -
A4C39_RS28070 53014..54180 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..92726 92726


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T66727 WP_001159868.1 NZ_CP017445:49078-49383 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T66727 NZ_CP017445:49078-49383 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT66727 WP_000813634.1 NZ_CP017445:48858-49076 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT66727 NZ_CP017445:48858-49076 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References