Detailed information of TA system TA066678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2510163..2510634
• Replicon: chromosome
• Accession: NZ_CP017442
• Organism: Escherichia coli O157:H7 strain 4276

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: A4C51_RS12895
• Protein ID: WP_001303511.1
• Coordinates: 2510163..2510441 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: A4C51_RS12900
• Protein ID: WP_001302048.1
• Coordinates: 2510443..2510634 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4C51_RS30870	2505169..2505353	-	185	Protein_2432	DUF3596 domain-containing protein	-
A4C51_RS12860	2505388..2505639	-	252	WP_000005552.1	excisionase family protein	-
A4C51_RS12865	2505712..2508183	-	2472	WP_070080217.1	exonuclease	-
A4C51_RS12870	2508276..2508467	-	192	WP_001090200.1	DUF1482 family protein	-
A4C51_RS12875	2508464..2508652	-	189	WP_000449192.1	cell division inhibition protein DicB	-
A4C51_RS12880	2509221..2509439	-	219	WP_001171930.1	hypothetical protein	-
A4C51_RS12885	2509511..2509810	-	300	WP_001240334.1	hypothetical protein	-
A4C51_RS12895	2510163..2510441	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A4C51_RS12900	2510443..2510634	-	192	WP_001302048.1	hypothetical protein	Antitoxin
A4C51_RS29040	2510655..2511026	-	372	WP_001169686.1	hypothetical protein	-
A4C51_RS12910	2511124..2511426	+	303	WP_000172738.1	transcriptional regulator	-
A4C51_RS12915	2511423..2511848	+	426	WP_000693943.1	Rha family transcriptional regulator	-
A4C51_RS12920	2511871..2512833	+	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
A4C51_RS12925	2512840..2513580	+	741	WP_000788938.1	ATP-binding protein	-
A4C51_RS12935	2513606..2514375	+	770	Protein_2446	DUF1627 domain-containing protein	-
A4C51_RS12940	2514391..2514786	+	396	WP_001118159.1	DUF977 family protein	-
A4C51_RS12945	2514843..2515427	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI	2501876..2551264	49388

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T66678 WP_001303511.1 NZ_CP017442:c2510441-2510163 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T66678 NZ_CP017442:c2510441-2510163 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT66678 WP_001302048.1 NZ_CP017442:c2510634-2510443 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT66678 NZ_CP017442:c2510634-2510443 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0