Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 359373..360004 Replicon chromosome
Accession NZ_CP017442
Organism Escherichia coli O157:H7 strain 4276

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag A4C51_RS01740 Protein ID WP_001259385.1
Coordinates 359373..359648 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag A4C51_RS01745 Protein ID WP_000593555.1
Coordinates 359645..360004 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4C51_RS01725 354377..355444 + 1068 WP_000361458.1 HlyD family secretion protein -
A4C51_RS01730 355441..358176 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
A4C51_RS01735 358176..359300 + 1125 WP_001301659.1 ABC transporter permease -
A4C51_RS01740 359373..359648 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
A4C51_RS01745 359645..360004 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
A4C51_RS01750 360054..360914 - 861 WP_001131788.1 class II aldolase -
A4C51_RS01755 360946..361215 - 270 WP_000933060.1 HPr family phosphocarrier protein -
A4C51_RS01760 361205..362713 - 1509 WP_001301773.1 carbohydrate kinase -
A4C51_RS01765 362706..364064 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
A4C51_RS01770 364141..364422 - 282 WP_000084026.1 PTS sugar transporter subunit IIB -
A4C51_RS01775 364419..364892 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 350517..360004 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T66662 WP_001259385.1 NZ_CP017442:359373-359648 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T66662 NZ_CP017442:359373-359648 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT66662 WP_000593555.1 NZ_CP017442:359645-360004 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT66662 NZ_CP017442:359645-360004 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References