Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 48867..49392 Replicon plasmid pO157
Accession NZ_CP017441
Organism Escherichia coli O157:H7 strain 3384

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag A4C38_RS28060 Protein ID WP_001159868.1
Coordinates 49087..49392 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag A4C38_RS28055 Protein ID WP_000813634.1
Coordinates 48867..49085 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4C38_RS30665 44150..44380 + 231 WP_010891288.1 hypothetical protein -
A4C38_RS28020 44501..45241 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
A4C38_RS28025 45526..46503 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
A4C38_RS28035 46911..47111 - 201 WP_000708307.1 hypothetical protein -
A4C38_RS28040 47108..47728 - 621 WP_001248529.1 hypothetical protein -
A4C38_RS28045 47725..48408 - 684 WP_010891291.1 DNA-binding protein -
A4C38_RS28055 48867..49085 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
A4C38_RS28060 49087..49392 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
A4C38_RS28065 49393..50199 + 807 WP_000016989.1 site-specific integrase -
A4C38_RS30675 52097..52435 + 339 WP_071525396.1 RepB family plasmid replication initiator protein -
A4C38_RS28080 53023..54189 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..92729 92729


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T66660 WP_001159868.1 NZ_CP017441:49087-49392 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T66660 NZ_CP017441:49087-49392 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT66660 WP_000813634.1 NZ_CP017441:48867-49085 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT66660 NZ_CP017441:48867-49085 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References