Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4601788..4602338 Replicon chromosome
Accession NZ_CP017436
Organism Escherichia coli O157:H7 strain 2149

Toxin (Protein)


Gene name ccdB Uniprot ID D3H432
Locus tag A4C44_RS23685 Protein ID WP_001160975.1
Coordinates 4601788..4602102 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag A4C44_RS23690 Protein ID WP_000125566.1
Coordinates 4602105..4602338 (-) Length 78 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4C44_RS23660 4597463..4598749 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
A4C44_RS23665 4598749..4599738 + 990 WP_000241259.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
A4C44_RS23670 4599735..4600556 + 822 WP_001065380.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
A4C44_RS23675 4600559..4600936 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
A4C44_RS23680 4600943..4601791 + 849 WP_000257173.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
A4C44_RS23685 4601788..4602102 - 315 WP_001160975.1 CcdB family protein Toxin
A4C44_RS23690 4602105..4602338 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
A4C44_RS23695 4602424..4602903 - 480 WP_000624372.1 type 3 dihydrofolate reductase -
A4C44_RS23700 4603095..4604957 - 1863 WP_000377184.1 glutathione-regulated potassium-efflux system protein KefC -
A4C44_RS23705 4604950..4605480 - 531 WP_000600700.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
A4C44_RS23710 4605588..4606919 - 1332 WP_001183177.1 MFS transporter -
A4C44_RS23715 4606977..4607264 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-103)

Antitoxin

(8-77)


Sequences


Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.76 Da        Isoelectric Point: 9.8517

>T66587 WP_001160975.1 NZ_CP017436:c4602102-4601788 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T66587 NZ_CP017436:c4602102-4601788 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT66587 WP_000125566.1 NZ_CP017436:c4602338-4602105 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT66587 NZ_CP017436:c4602338-4602105 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References