Detailed information of TA system TA066578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2732301..2732939
• Replicon: chromosome
• Accession: NZ_CP017436
• Organism: Escherichia coli O157:H7 strain 2149

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: A4C44_RS14190
• Protein ID: WP_000813794.1
• Coordinates: 2732763..2732939 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: A4C44_RS14185
• Protein ID: WP_001270286.1
• Coordinates: 2732301..2732717 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4C44_RS14165	2727453..2728394	-	942	WP_001251315.1	ABC transporter permease	-
A4C44_RS14170	2728395..2729408	-	1014	WP_000220402.1	ABC transporter ATP-binding protein	-
A4C44_RS14175	2729426..2730571	-	1146	WP_000047432.1	ABC transporter substrate-binding protein	-
A4C44_RS14180	2730816..2732222	-	1407	WP_000760654.1	PLP-dependent aminotransferase family protein	-
A4C44_RS14185	2732301..2732717	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
A4C44_RS14190	2732763..2732939	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
A4C44_RS14195	2733161..2733391	+	231	WP_000494244.1	YncJ family protein	-
A4C44_RS14200	2733483..2735444	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
A4C44_RS14205	2735517..2736053	-	537	WP_000429145.1	DNA-binding transcriptional regulator SutR	-
A4C44_RS14210	2736106..2737317	+	1212	WP_071525382.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T66578 WP_000813794.1 NZ_CP017436:c2732939-2732763 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T66578 NZ_CP017436:c2732939-2732763 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT66578 WP_001270286.1 NZ_CP017436:c2732717-2732301 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT66578 NZ_CP017436:c2732717-2732301 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB