Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 940187..940841 Replicon chromosome
Accession NZ_CP017436
Organism Escherichia coli O157:H7 strain 2149

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag A4C44_RS04825 Protein ID WP_000244781.1
Coordinates 940434..940841 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag A4C44_RS04820 Protein ID WP_000354046.1
Coordinates 940187..940453 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4C44_RS04800 936275..937708 - 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -
A4C44_RS04805 937753..938064 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
A4C44_RS04810 938228..938887 + 660 WP_000250274.1 hemolysin III family protein -
A4C44_RS04815 938964..939944 - 981 WP_000886053.1 tRNA-modifying protein YgfZ -
A4C44_RS04820 940187..940453 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
A4C44_RS04825 940434..940841 + 408 WP_000244781.1 protein YgfX Toxin
A4C44_RS04830 940881..941402 - 522 WP_001055874.1 flavodoxin FldB -
A4C44_RS04835 941514..942410 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
A4C44_RS04840 942435..943145 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
A4C44_RS04845 943151..944884 + 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T66565 WP_000244781.1 NZ_CP017436:940434-940841 [Escherichia coli O157:H7]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T66565 NZ_CP017436:940434-940841 [Escherichia coli O157:H7]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT66565 WP_000354046.1 NZ_CP017436:940187-940453 [Escherichia coli O157:H7]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT66565 NZ_CP017436:940187-940453 [Escherichia coli O157:H7]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References