Detailed information of TA system TA066540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2513800..2514271
• Replicon: chromosome
• Accession: NZ_CP017434
• Organism: Escherichia coli O157:H7 strain 1130

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: A4C50_RS12915
• Protein ID: WP_001303511.1
• Coordinates: 2513800..2514078 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: A4C50_RS12920
• Protein ID: WP_001302048.1
• Coordinates: 2514080..2514271 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4C50_RS30960	2508806..2508990	-	185	Protein_2439	DUF3596 domain-containing protein	-
A4C50_RS12880	2509025..2509276	-	252	WP_000005552.1	excisionase family protein	-
A4C50_RS12885	2509349..2511820	-	2472	WP_070080217.1	exonuclease	-
A4C50_RS12890	2511913..2512104	-	192	WP_001090200.1	DUF1482 family protein	-
A4C50_RS12895	2512101..2512289	-	189	WP_000449192.1	cell division inhibition protein DicB	-
A4C50_RS12900	2512858..2513076	-	219	WP_001171930.1	hypothetical protein	-
A4C50_RS12905	2513148..2513447	-	300	WP_001240334.1	hypothetical protein	-
A4C50_RS12915	2513800..2514078	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A4C50_RS12920	2514080..2514271	-	192	WP_001302048.1	hypothetical protein	Antitoxin
A4C50_RS29115	2514292..2514663	-	372	WP_001169686.1	hypothetical protein	-
A4C50_RS12930	2514761..2515063	+	303	WP_000172738.1	transcriptional regulator	-
A4C50_RS12935	2515060..2515485	+	426	WP_000693943.1	Rha family transcriptional regulator	-
A4C50_RS12940	2515508..2516470	+	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
A4C50_RS12945	2516477..2517217	+	741	WP_000788938.1	ATP-binding protein	-
A4C50_RS12955	2517243..2518012	+	770	Protein_2453	DUF1627 domain-containing protein	-
A4C50_RS12960	2518028..2518423	+	396	WP_001118159.1	DUF977 family protein	-
A4C50_RS12965	2518480..2519064	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI	2505513..2554907	49394

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T66540 WP_001303511.1 NZ_CP017434:c2514078-2513800 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T66540 NZ_CP017434:c2514078-2513800 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT66540 WP_001302048.1 NZ_CP017434:c2514271-2514080 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT66540 NZ_CP017434:c2514271-2514080 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0