Detailed information of TA system TA066516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 983191..983705
• Replicon: chromosome
• Accession: NZ_CP017428
• Organism: Staphylococcus simulans strain MR3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BI282_RS04460
• Protein ID: WP_031262808.1
• Coordinates: 983355..983705 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A2T4QNU3
• Locus tag: BI282_RS04455
• Protein ID: WP_023016097.1
• Coordinates: 983191..983358 (+)
• Length: 56 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BI282_RS13980	979249..979545	+	297	WP_158263689.1	PH domain-containing protein	-
BI282_RS04435	979538..981031	+	1494	WP_105967037.1	PH domain-containing protein	-
BI282_RS04440	981024..981521	+	498	WP_023016100.1	PH domain-containing protein	-
BI282_RS04445	981523..981876	+	354	WP_031262810.1	holo-ACP synthase	-
BI282_RS04450	981956..983110	+	1155	WP_105967038.1	alanine racemase	-
BI282_RS04455	983191..983358	+	168	WP_023016097.1	hypothetical protein	Antitoxin
BI282_RS04460	983355..983705	+	351	WP_031262808.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BI282_RS04465	983997..984998	+	1002	WP_106420981.1	PP2C family protein-serine/threonine phosphatase	-
BI282_RS04470	985074..985400	+	327	WP_105967039.1	anti-sigma factor antagonist	-
BI282_RS04475	985402..985881	+	480	WP_023016096.1	anti-sigma B factor RsbW	-
BI282_RS04480	985856..986626	+	771	WP_002481747.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12976.09 Da
• Isoelectric Point: 10.0474

>T66516 WP_031262808.1 NZ_CP017428:983355-983705 [Staphylococcus simulans]
MMRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TVDKKRLKEKLTYLSDEKMKEIDNAIGISLGLTHKN

Nucleotide

• Length: 351 bp

>T66516 NZ_CP017428:983355-983705 [Staphylococcus simulans]
ATGATGAGGAGAGGCGATGTTTATTTAGCTGATTTATCACCAGTGCAAGGTTCTGAACAAGGGGGAGTTAGACCTGTTGT
AATTATACAAAATGACACTGGCAATAAGTACAGTCCGACGGTAATTGTAGCCGCGATTACTGGGCGTATTAATAAGGCGA
AAATTCCAACACATGTTGAAATTGAAAAGAGTAAATACAAATTAGACAAAGATTCTGTTATATTACTTGAACAGATTAGA
ACAGTTGATAAAAAACGTTTGAAAGAGAAATTGACTTATCTTTCAGATGAGAAGATGAAAGAAATTGATAACGCGATTGG
AATTAGTTTAGGCTTGACACATAAAAATTAG

Antitoxin

Protein

• Length: 56 a.a.
• Molecular weight: 6257.78 Da
• Isoelectric Point: 3.6836

>AT66516 WP_023016097.1 NZ_CP017428:983191-983358 [Staphylococcus simulans]
MSTFNQNRSYSLEQSLKEGYSQMADLNLSLATEAFSVECEACDCNESYLTFDKDE

Nucleotide

• Length: 168 bp

>AT66516 NZ_CP017428:983191-983358 [Staphylococcus simulans]
ATGTCAACTTTTAATCAGAATAGAAGCTATAGTTTGGAACAGTCATTAAAAGAAGGCTATTCACAAATGGCTGATTTGAA
TCTCTCCCTAGCAACGGAGGCATTTTCAGTGGAATGTGAAGCCTGTGATTGCAATGAATCTTATCTTACATTTGATAAGG
ATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2T4QNU3